DIG Northern Starter Kit

Description

• General description: The DIG Northern Starter Kit is designed for the novice DIG system user. It contains the reagents proven to provide successful, reproducible results in northern blots. Additionally, the more convenient forms of standard DIG system products are included (e.g., CDP Star, ready-to-use). The small number of reactions allows the user to gain a firm foundation using the DIG system, then "graduate" to the standard pack sizes. For optimum success, use this kit with the DIG Wash and Block Buffer Set.This kit is used for the generation of single-stranded, DIG-labeled RNA probes and chemiluminescent detection. Labeled probes are synthesized by the in vitro transcription method.
• Application: DIG northern starter kit has been used in northern blot hybridization to analyse hepatitis B virus (HBV) RNAs, citrus leprosis virus cytoplasmic type 2 (CiLV-C2) RNAs.[1][2]
• Packaging: 1 kit containing 11 components.1 kit for up to 10 labeling reactions and detection of 10 blots, blots of 10×10cm2, reactions of μg DNA, yielding approx. 20μg labeled RNA, each
• Preparation Note: Working solution: Note: Use sterile, RNase-free solutions and equipmentAssay Time: 9 hours 30 minutesSample Materials:Linearized plasmid, including the appropriate RNA polymerase promoter sequence (SP6, T3, T7).Specially prepared PCR productWorking solution: Note: Use sterile, RNase-free solutions and equipment.Note: The length of the region to be transcribed should be in the range of 200 to 1,000 bp. To avoid RNase contamination the DNA must be phenolized.
• Other Notes: For life science research only. Not for use in diagnostic procedures.