DIG Nucleic Acid Detection Kit

Description

• General description: DIG Nucleic Acid Detection Kit is used for detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate).
• Application: DIG nucleic acid detection kit has been used in the confirmation of genetic manipulation.[1]
• Features and Benefits: We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
• Packaging: 1 kit containing 5 components.
• Principle: DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP antibody conjugate and the color substrates NBT and BCIP.
• Preparation Note: Working concentration: Flow cytometry: 2 μg/ml (0.2 μg/100 μl/106 cells); Immunohistocytochemistry: 6 μg/mlWorking concentration of conjugate depends on application and substrate.Storage conditions (working solution): Anti-Digoxigenin-AP Conjugate (vial 3): 2 to 8 °C, it is stable for 12 months at this temperatureNBT/BCIP (vial 4): 2 to 8 °C, stableNote: During shipment of the kit on dry ice, a precipitate may occur which is dissolved by briefly warming to 37 °CThe blocking reagent (vial 5) is stable for 36 months and can be stored dry at 4 to 8 °C.Autoclaved stock solution can be stored for several days to a week either unopened at 15 to 25 °C or at 4 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.
• Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research