Biotin Protein Labeling Kit

Description

• General description: For labeling of proteins with biotin.Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.
• Application: Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates.[1]The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.[2]
• Packaging: 1 kit containing 5 components.
• Quality: Function tested.
• Preparation Note: Working solution: Preparation of the solutionsBlocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).
• Other Notes: For life science research only. Not for use in diagnostic procedures.