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11681451001

NBT/BCIP Stock Solution

Name: NBT/BCIP Stock Solution | Sigma Aldrich | Chemikart
Brand: Chemikart

Manufacturer: Sigma Aldrich

Synonym(S): 5-bromo-4-chloro-3-indoyl phosphate, BCIP

Select a Size

Pack Size	SKU	Availability	Price
8 ML	11681451001-8-ML	In Stock	₹ 11,177.70

11681451001 - 8 ML

₹ 11,177.70

In Stock

Quantity

Base Price: ₹ 11,177.70

GST (18%): ₹ 2,011.986

Total Price: ₹ 13,189.686

form

solution

Quality Level

100

mol wt

817.7

packaging

pkg of 8 mL

manufacturer/tradename

Roche

storage temp.

2-8°C

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Description

General description: The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9NEvaluation: visually
Application: The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blotNorthern blotWestern blot[1]Colony and plaque liftsIn situ hybridization[2][3]and for applications in immunohistochemistry and immunocytochemistry.Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes.
Physical form: Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v).Note: The color of the solution can vary from yellowish to light brown.
Preparation Note: Working solution: Preparation of staining solutionAlways prepare this solution fresh shortly before use. For all applications except DIG System: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.For DIG System applications: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaClNote: Do not include MgCl2 in the detection buffer as this may lead to spotty background on the membrane after the detection procedure. Insoluble in water.
Other Notes: For life science research only. Not for use in diagnostic procedures.

SAFETY INFORMATION

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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NBT/BCIP Stock Solution

Select a Size

Select a Size

Properties

form

Quality Level

mol wt

packaging

manufacturer/tradename

storage temp.

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Description

SAFETY INFORMATION

WGK

Flash Point(F)

Flash Point(C)

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Sigma Aldrich

form:

solution

Quality Level:

100

mol wt:

817.7

packaging:

pkg of 8 mL

manufacturer/tradename:

Roche

storage temp.:

2-8°C

Tocris Bioscience™

form:

Quality Level:

mol wt:

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manufacturer/tradename:

storage temp.:

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form:

solution (ready-to-use)

Quality Level:

100

mol wt:

__

packaging:

pkg of 3 × 100 mL

manufacturer/tradename:

Roche

storage temp.:

2-8°C

Sigma Aldrich

form:

solution

Quality Level:

100

mol wt:

__

packaging:

pkg of 40 μL

manufacturer/tradename:

Roche

storage temp.:

−20°C

NBT/BCIP Stock Solution

Select a Size

Select a Size

Properties

form

Quality Level

mol wt

packaging

manufacturer/tradename

storage temp.

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Sigma Aldrich

Sigma Aldrich

Sigma Aldrich

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Description

SAFETY INFORMATION

WGK

Flash Point(F)

Flash Point(C)

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Sigma Aldrich

form: solution Quality Level: 100 mol wt: 817.7 packaging: pkg of 8 mL manufacturer/tradename: Roche storage temp.: 2-8°C

Tocris Bioscience™

form: __ Quality Level: __ mol wt: __ packaging: __ manufacturer/tradename: __ storage temp.: __

Sigma Aldrich

form: solution (ready-to-use) Quality Level: 100 mol wt: __ packaging: pkg of 3 × 100 mL manufacturer/tradename: Roche storage temp.: 2-8°C

Sigma Aldrich

form: solution Quality Level: 100 mol wt: __ packaging: pkg of 40 μL manufacturer/tradename: Roche storage temp.: −20°C

form:

solution

Quality Level:

100

mol wt:

817.7

packaging:

pkg of 8 mL

manufacturer/tradename:

Roche

storage temp.:

2-8°C

form:

Quality Level:

mol wt:

packaging:

manufacturer/tradename:

storage temp.:

form:

solution (ready-to-use)

Quality Level:

100

mol wt:

__

packaging:

pkg of 3 × 100 mL

manufacturer/tradename:

Roche

storage temp.:

2-8°C

form:

solution

Quality Level:

100

mol wt:

__

packaging:

pkg of 40 μL

manufacturer/tradename:

Roche

storage temp.:

−20°C