CSPD ready-to-use

Description

• General description: CSPD is a chemiluminescent substrate for alkaline phosphatase that enables extremely fast and sensitive detection of biomolecules by producing visible light. Light emission is recorded on X-ray film or on luminescence imager systems.[1]
• Application: CSPD ready-to-use has been used for:the nonradioactive detection of labeled nucleic acid probes on membrane blots (Southern blot, Northern blot, Western blot and Dot blot)[2][3]colony and plaque hybridizationgel shift assaysequencingIt may be used for the detection of alkaline phosphatase and alkaline phosphatase conjugates either in solution or on solid supports.
• Features and Benefits: Save time with a fast, highly sensitive substrate.Easily strip and reprobe membranes.Take multiple exposures of up to two days for each experiment.Reuse the substrate by filtering and storing in sodium azide.
• Physical form: 25mM solution (11.6mg/ml), 100x concentrated, colorless solution.
• Preparation Note: Storage conditions (working solution): Washing Buffer: 15 to 25 °CMaleic Acid Buffer: 15 to 25 °CDetection Buffer: 15 to 25 °CBlocking Stock Solution, 10x: 2 to 8 °CBlocking Solution: always prepare freshAntibody Solution: 2 to 8 °C
• Other Notes: For life science research only. Not for use in diagnostic procedures.Purity: Purified by HPLCDevelopment time: VariableNote: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.Activated by preincubation (10 minutes at +37°C).Apply substrate quickly to avoid drying of membrane (background).Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.Do not use nitrocellulose membranes.Repeat exposures can be made several days after addition of substrate.