High Pure miRNA Isolation Kit

Description

• General description: In the presence of a chaotropic salt, RNA binds selectively to special glass fibers prepacked in the High Pure Spin Filter Tube. Bound RNA is purified in a series of rapid wash-and-spin steps to remove contaminating salts, proteins, and other cellular impurities, and then eluted using a low-salt solution. By lowering the concentration of Binding Enhancer during the binding step in the two-column protocol, the small RNA-containing miRNA passes the first column unbound. When the concentration of Binding Enhancer is increased, the small RNA fraction can be bound to a second High Pure Spin Filter Tube.
• Application: The High Pure miRNA Isolation kit rapidly purifies RNA that is suitable for direct use in many downstream applications:Northern blottingcDNA synthesisPrimer extensionmiRNA array hybridizationRelative quantification of miRNA with RT-PCR using, for example, the LightCycler® 480 System
• Features and Benefits: Isolate DNA-free miRNA ideal for qualitative and quantitative reverse transcription. ideal for qualitative and quantitative reverse transcription.Obtain excellent performance and linearity in RT-PCR.Generate stable , highly pure miRNAs using a simple, efficient protocol.Purify miRNA without using hazardous organic solvents. Avoid phenol/chloroform steps required by other suppliers′ miRNA purification kits.Choose one flexible kit for all your miRNA purifications. Use the same kit to purify small RNAs from a variety of sample types.
• Components: Tissue Lysis Buffer (for FFPE sections)Proteinase K, recombinant (for FFPE sections)Binding BufferBinding EnhancerWash BufferElution BufferHigh Pure Filter TubesCollection Tubes
• Quality: More than 60% recovery is obtained when 1 μg miRNA is added to106 K-562 cells and isolated with the one-column method, and more than 50% is recovered with the two-column method.
• Other Notes: High Pure Technology and Silica Adsorption Kits
• Legal Information: LightCycler is a registered trademark of Roche