GenElute™ 96 Well Total RNA Purification Kit

Description

• General description: GenElute 96 Well Total RNA Purification Kit allows for the high-throughput purification of total RNA from cells or tissue. The kit utilizes the advantages of silica membrane technology and eliminates the use of hazardous organic compounds such as phenol and chloroform.
• Application: The purified total RNA is ready for use in any enzymatic downstream application.
• Features and Benefits: Unique plate design offers low risk of cross-contaminationRNA can be used for any kind of downstream enzymatic reactionProcessing possible under with a vacuum or centrifugeSuitable for manual and automated processing
• Principle: Sigma′s GenElute 96 Well Total RNA Purification Kit provides a simple and convenient way to isolate total RNA from cells or tissue. The cells or tissue are lysed in a solution containing large amounts of chaotropic salt which immediately inactivates RNases that are present in almost all biological materials. The addition of a Wash Buffer provides the necessary binding conditions for the RNA to be adsorbed to the silica membrane. The contaminating DNA which may be bound to the silica membrane is removed by directly applying RNase-free DNase. The remaining salts, proteins, and other cellular debris are removed by additional washing steps. The final step is to elute the purified RNA in the RNAase-free water that is supplied in the kit.
• Other Notes: Application notes:Method of purification:Silica-membrane technologyCompatible with Manual or automated, vacuum or centrifugationEfficient recovery of > 200 ntTypical yield20 μg (2 x 106 HeLa cells),20 μg (20 mg mouse liver)Typical concentration50–200 ng/μLElution volume50–130 μLPreparation time70 min/plateBinding capacity100 μgRNA ratio28S/18S approx. 2.1A260/A2801.90–2.10Typical RIN (RNA > 9 (cells)integrity number)≥ 7 (tissue)Sufficient for:< 107 cultured cells (centrifugation)< 30 mg tissue (centrifugation)saliva Related products: Prod #Related prod #RTN9604RTN9602RTN9602RTN9604
• Legal Information: GenElute is a trademark of Sigma-Aldrich Co. LLC