REDExtract-N-Amp™ Blood PCR Kit

Description

• General description: The REDExtract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR (polymerase chain reaction). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification. The REDExtract-N-Amp Blood PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
• Application: REDExtract-N-Amp™ Blood PCR Kit has been used to extract genomic DNA from blood samples, peripheral blood leukocytes, parasites and endocervical swab eluates of mice.[1][2][3][4][5][6] It has also been used to perform polymerase chain reaction (PCR) using oligonucleotide primers.[7]
• Features and Benefits: Efficient 8 minute prep allows greater speed and throughputNo need for organic extraction, centrifugation or alcohol precipitationHot start antibody for highly specific PCR amplification of genomic DNACan be used with whole blood or blood cardsExtract stable at 4 °C for at least 6 monthsCompatible with any format (single tube, 96-well, etc.) Simple, 3 step procedure with no special equipment required REDExtract-N-Amp™ requires no loading buffers or tracking dyes required for gel analysisPCR products are suitable for direct cloning and sequencing
• Principle: Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.
• Other Notes: For additional information, please see www.sigma-aldrich.com/extract-n-amp.
• Legal Information: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.