REDExtract-N-Amp™ Plant PCR Kit

Description

• General description: The REDExtract-N-Amp™ Plant PCR Kits enable the rapid detection of plant pathogens by direct PCR (polymerase chain reaction). These kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by direct PCR. The REDExtract-N-Amp PCR ReadyMix™ contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
• Application: REDExtract-N-Amp™ Plant PCR Kit has been used to: extract genomic DNA from mycelial lawns and leaves[1][2][3]screen leaf tissue from putative transgenic potato plants[4]perform polymerase chain reaction (PCR)[5][6][7][8]isolate DNA from Cannabis sativa L. seed[9] and dried fruit body gills[10]
• Features and Benefits: Single-step extraction of plant genomic DNA for PCR in less than 15 minutesNo freezing, mechanical disruption, organic extraction, column purification or precipitation required Specially formulated PCR ReadyMix for use with extractHot Start antibody for highly specific PCR amplification of genomic DNAREDExtract-N-Amp™ requires no loading buffers or tracking dyes required for gel analysisCompatible with high-throughput requirements for genetic analysis of plantsExtract stable at 4 °C for at least 6 months
• Components: Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix.
• Other Notes: For additional information, please see www.sigma-aldrich.com/extract-n-amp.
• Legal Information: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.