KAPA2G Fast PCR Kit

Description

• General description: KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets. KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
• Application: KAPA2G Fast PCR Kit has been used in:PCR with confronting 2 -pair primers assay (PCR-CTPP)[1][2]Routine PCR[3]Fast PCR[4]Genotyping
• Biochem/physiol Actions: KAPA2G Fast DNA Polymerase generated DNA fragments is useful in sequencing restriction enzyme digestion and cloning experiments. KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities like wild-type Taq, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.
• Features and Benefits: Reduce cycling times up to 75% :Extension times as low as 1 sec per kilobaseBroad coverage of both AT- and GC-rich targetsHigh speed, higher performance :Single copy sensitivity from complex targetsIncreased yieldsQuick Notes :KAPA2G Fast PCR Kits with dNTPs contain the engineered KAPA2G Fast DNA Polymerase, developed for fast PCR.Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.No need for specialized instrumentation or PCR consumables.Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.Use 0.5 U KAPA2G Fast DNA Polymerase per 25 μL reaction, or less for smaller volumes.For high reaction efficiency, do not exceed 25 μL reaction volumes.KAPA2G Buffer A contains 1.5 mM MgCl2 at 1X.Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.
• Quality: Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Fast Ready Mix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.
• Preparation Note: Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.
• Other Notes: For Research Use Only. Not for use in diagnostic procedures.