KOD Xtreme Hot Start DNA Polymerase

Description

• General description: *Manufactured by Toyobo and distributed by EMD Millipore. Not available from Merck KGaA, Darmstadt, Germany in Japan.Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
• Features and Benefits: Optimized for the highest PCR success rate, even with the most difficult targets.10X higher fidelity than Taq blends Efficiently amplifies up to 90% GC-content DNA templates Amplifies genomic targets up to 24 kbp Amplifies phage/Lambda targets up to 40 kbp Eliminates mispriming and primer-dimer formation Convenient ambient-temperature setup compatible with automation
• Components: 1 × 200 U KOD Xtreme<TMSYMBOL></TMSYMBOL> Hot Start DNA Polymerase3 × 1.7 mL 2X Xtreme Buffer2 × 1 mL dNTPs (2 mM each)
• Warning: Toxicity: Irritant (B)
• Unit Definition: One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA.
• Legal Information: NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany