KOD One™ PCR Master Mix

Description

• General description: KOD One™ PCR master Mix is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/kb for template DNA <10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples, it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.
• Application: Genomic DNA amplification[1][2]cDNA amplificationDirect PCRColony PCRAmplification of NGS librariesSite direct gene mutationCompatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.Sample Volume Needed: about 50ng DNA
• Features and Benefits: Fast: Amplifies the targets using the following very short conditions: o =1 kb: 1 sec o 1~ 10 kb: 5 sec/ kb o 10 kb~: 10 sec/ kbReady-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl2.High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymeraseHigh Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)
• Packaging: KMM-101NV - 5X1ML is sufficient for 500 reactions at 20 μL each or for 200 reactions at 50 μL each.KMM-101NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.
• Other Notes: For R&D use only. Not for drug, household, or other uses.
• Legal Information: *Manufactured by Toyobo and distributed by Sigma-Aldrich. Not available in Japan.