Enhanced Avian First Strand Synthesis Kit

Description

• General description: Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)+ RNA.
• Application: Enhanced Avian First Strand Synthesis Kit has been used for cDNA synthesis, which is intended for use in a real-time reverse transcription-polymerase chain reaction (RT-PCR).[1][2]
• Unit Definition: One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)12-18 as primer.
• Legal Information: Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.