JumpStart™ Taq DNA Polymerase

Description

• General description: The Taq DNA polymerase activity is inactivated by combining the enzyme with JumpStart™ Taq antibody, a neutralizing monoclonal antibody to Taq DNA polymerase. Antibody inactivation provides a simple, efficient procedure for hot-start polymerase chain reaction (PCR). Hot start PCR can significantly improve the results of DNA amplification by reducing the generation of nonspecific amplification products and primer-dimer artifacts. When used in PCR, JumpStart Taq DNA polymerase is inactive at low (room) temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active.
• Application: JumpStart™ Taq DNA polymerase has been used: In the PCR amplification of DNA isolated from herbarium specimens.[1] In the PCR reaction mixture for randomly amplified polymorphic DNA polymerase chain reaction (RAPD PCR).[2] To amplify and detect a point mutation in the EGFR exon 19 using specific cancer cell lines.[3] In real-time quantitative PCR[4] For PCR amplification of complex genomic or cDNA templatesFor the PCR amplification of very low-copy number targetsFor the PCR amplification of many thermal cycles (>35), and multiple primer pairs in the same reaction tube
• Features and Benefits: Reduces non-specific amplificationIncreases PCR specificity and yieldReduces set-up time concerns associated with manual or wax Hot Start methodsActivation time of less than 1 minute
• Packaging: JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl2 for optimization.
• Other Notes: Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
• Unit Definition: One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
• Legal Information: Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.