AccuTaq™ LA DNA Polymerase

Description

• General description: AccuTaq™ long and accurate (LA) DNA Polymerase is a combination of Taq DNA polymerase with a small amount of a polymerase with the 3′→5′ exonuclease activity necessary for proofreading. It enables the amplification of genomic targets larger than 20 kb. This blend increases the length of amplification products by using the proofreading polymerase to repair terminal misincorporations. This repair enables the polymerase to resume elongating the growing DNA strand. After the proofreading activity, the final products mostly have a blunt end.
• Application: AccuTaq™ LA DNA Polymerase produces long DNA amplicons for:Mutation analysisCloning genesDNA sequencingcDNA library generationPolymerase chain reaction (PCR)[1][2]Direct PCR[3]
• Features and Benefits: Increased fidelity, up to 6.5× that of Taq DNA polymeraseEfficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA.
• Packaging: The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates. A separate vial of DMSO is included. Addition of DMSO in the reaction at a final concentration of 1-4% may increase yield and improve reliability of the system with some complex PCR targets.
• Other Notes: AccuTaq LA DNA polymerase is an optimized blend of Sigma′s high quality Taq DNA polymerase and a small amount of an additional polymerase that exhibits 3′→5′ exonuclease or proofreading activity. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate.
• Unit Definition: One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74°C.
• Legal Information: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.