KAPA PROBE FORCE

Description

• General description: KAPA PROBE FORCE is a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. The master mix contains a third generation DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Crude samples can now be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA using KAPA PROBE FORCE.Direct qPCR from crude blood, tissue, and plant extractsSample-to-Cq workflows in <1 hourHigh efficiency for accurate, reproducible, and sensitive resultsSuperior tolerance to carry-over inhibitorsMultiplex compatibility with crude extracts
• Application: KAPA PROBE FORCE has been used for:GMO testingMouse transgenicsSNP genotyping[1]Food/water pathogen detectionInfectious disease researchCancer researchDNA quantification[2]Quantitative polymerase chain reaction (qPCR)[3]Digital droplet PCR[4]For amplification of templates directly from cDNA synthesis reactions
• Features and Benefits: Streamline sample-to-Cq workflows in <1 hour:Eliminate the time and cost of sample purification by amplifying directly from crude samplesAnalyze a wide range of sample types including whole blood, cells, mouse tails, FFPE, leaf, stem, seed, and soilGenerate accurate and reproducible results:Kits include a third-generation DNA polymerase, evolved for robust target amplification and detectionEnzyme maintains high reaction efficiency in the presence of PCR inhibitors for reliable data generationBreak through high levels of qPCR inhibitors:Achieve greater levels of sensitivity for inhibited blood, tissue, and plant samplesConvert purified DNA assays to crude workflows without observable Cq delaysMultiplex crude samples efficiently:Accelerate genotyping analysis with single reaction allelic discrimination of crude DNA extractsMaximize data collection from precious samples, increase throughput, and reduce costsQuick Notes:This kit contains the KAPA3G HotStart DNA Polymerase enzyme, enabling probe-based qPCR for both routine and challenging sample types.Initial denaturation of 3 min at 98°C is recommended to ensure complete denaturation of complex target DNA. A 5-min denaturation time may be required for some crude samples.For two-step cycling, use a 20-sec combined annealing/extension/data acquisition at 60°C as a first approach.A 10-sec annealing/extension/data acquisition time may be used with most assays, but this must be determined empirically.For crude samples, the amount of sample in the reaction may be reduced to improve performance, but this must be determined empirically.
• Quality: KAPA PROBE FORCE qPCR Master Mix Universal is subjected to stringent functional quality control, free of detectable contaminating exo- and endonuclease activities, and meets strict requirements with respect to DNA contamination.
• Preparation Note: ROX reference dye is sensitive to light exposure. Always ensure that the product has been fully thawed and mixed before use. Avoid repeated freezing and thawing.
• Other Notes: For Research Use Only. Not for use in diagnostic procedures.