Cytiva Sera-Xtracta™ HMW DNA Kit

Description

• Sera-Xtracta™ HMW DNA Kit offers magnetic bead-based extraction and purification of genomic DNA from whole blood (treated with ethylenediaminetetraacetic acid (EDTA), citrate or heparin), buffy coat, saliva, cultured mammalian cells and solid tissue samples
• The extraction protocols are designed to efficiently remove contaminants and PCR inhibitors while minimizing shearing, resulting in high quality high-molecular-weight genomic DNA with minimal RNA carryover
• Suitable for long-read sequencing: Isolates high-molecular-weight DNA > 200 kb
• Efficient scale-up and flexibility: Provides robust chemistry for sample input volumes from 200 μL – 2 mL
• Efficient removal of inhibitors to give high purity genomic DNA: Typical purity ratio (A260/A280 and A260/A230) of > 1.7
• Automation friendly reagents and easy to use protocols Simplified protocol minimizes the co-purification of RNA
• Compatible with molecular biology techniques, including next-generation sequencing (NGS), cloning, restriction enzyme digestion, PCR amplification and genotyping applications
• The kit uses chaotropic agents to extract DNA from samples, denature protein components and promote the selective binding of DNA to the silica-coated magnetic beads
• Proteinase K is the protease of choice to digest protein from samples, because it is active even when enzyme inhibitors such as EDTA and detergents are present in samples
• Denatured contaminants are easily removed by subsequent washing of the silica beads with an ethanolic buffer set
• The purified genomic DNA is eluted in a low ionic strength buffer at a concentration suitable for most downstream molecular biology applications.