3676714
Cytiva plasmidPrep Mini Spin Kit
For the rapid isolation of high and low copy number plasmid DNA
Manufacturer: Fischer Scientific
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Description
Purify high yields of plasmid DNA in less than 10 min. Color-coded tubes and columns simplify the protocol and purified DNA is suitable for all common downstream applications.
Includes
Spin columns prepacked with a novel Silica membrane, Suspension, Lysis and Neutralization Buffers containing chaotropic salts, Wash and Elution Buffers, microcentrifuge collection tubes, full protocol booklet, Detachable, quick reference protocol card
Purity
1.7
Yield
6-15 μg
For Use With (Application)
Suitable for many downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCRSequencing, PCR, ligation and cloning, restriction digest, enzymatic modification, template for labeled probe generation, transforma
Product Type
illustra plasmidPrep Mini Spin Kit
Test Time
Approx. 9 min
Related Products
Description
- The illustra plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification
- Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane
- Total preparation time of less than 10 minutes—representing a 50% reduction in protocol time Produces high-quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing Increased ease of use provided minimal changes in pipetting volume from one part of the protocol to the next, less centrifugation steps, color-coded kit components, and lack of organic solvents The typical plasmid yields from a fresh 1.5 ml culture of E.coli containing a high copy number plasmid is approximately 9 μg Can accommodate high and low copy number plasmid purification
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Description: Purify high yields of plasmid DNA in less than 10 min. Color-coded tubes and columns simplify the protocol and purified DNA is suitable for all common downstream applications.
Includes: Spin columns prepacked with a novel Silica membrane, Suspension, Lysis and Neutralization Buffers containing chaotropic salts, Wash and Elution Buffers, microcentrifuge collection tubes, full protocol booklet, Detachable, quick reference protocol card
Purity: 1.7
Yield: 6-15 μg
For Use With (Application): Suitable for many downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCRSequencing, PCR, ligation and cloning, restriction digest, enzymatic modification, template for labeled probe generation, transforma
Product Type: illustra plasmidPrep Mini Spin Kit
Test Time: Approx. 9 min
Description:
Purify high yields of plasmid DNA in less than 10 min. Color-coded tubes and columns simplify the protocol and purified DNA is suitable for all common downstream applications.
Includes:
Spin columns prepacked with a novel Silica membrane, Suspension, Lysis and Neutralization Buffers containing chaotropic salts, Wash and Elution Buffers, microcentrifuge collection tubes, full protocol booklet, Detachable, quick reference protocol card
Purity:
1.7
Yield:
6-15 μg
For Use With (Application):
Suitable for many downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCRSequencing, PCR, ligation and cloning, restriction digest, enzymatic modification, template for labeled probe generation, transforma
Product Type:
illustra plasmidPrep Mini Spin Kit
Test Time:
Approx. 9 min
Description: illustra tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from a variety of animal tissues and mammalian cell cultures in just 90 min for tissues and 45 min for ce
Includes: Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card
Purity: 1.75
Yield: __
For Use With (Application): Used for several downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR
Product Type: illustra tissue and cells genomicPrep Mini Spin Kit
Test Time: 90 min[Animal Tissue], 45 min[Cultured cells]
Description:
illustra tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from a variety of animal tissues and mammalian cell cultures in just 90 min for tissues and 45 min for ce
Includes:
Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card
Purity:
1.75
Yield:
__
For Use With (Application):
Used for several downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR
Product Type:
illustra tissue and cells genomicPrep Mini Spin Kit
Test Time:
90 min[Animal Tissue], 45 min[Cultured cells]
Description: illustra tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from a variety of animal tissues and mammalian cell cultures in just 90 min for tissues and 45 min for ce
Includes: Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card
Purity: 1.75
Yield: __
For Use With (Application): Used for several downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR
Product Type: illustra tissue and cells genomicPrep Mini Spin Kit
Test Time: 90 min[Animal Tissue], 45 min[Cultured cells]
Description:
illustra tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from a variety of animal tissues and mammalian cell cultures in just 90 min for tissues and 45 min for ce
Includes:
Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card
Purity:
1.75
Yield:
__
For Use With (Application):
Used for several downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR
Product Type:
illustra tissue and cells genomicPrep Mini Spin Kit
Test Time:
90 min[Animal Tissue], 45 min[Cultured cells]
Description: __
Includes: __
Purity: __
Yield: __
For Use With (Application): __
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Includes:
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Purity:
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Yield:
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For Use With (Application):
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