Uses modified nucleotides in isothermal rolling circle amplification (RCA) to improve results with difficult to sequence templates
Manufacturer: Fischer Scientific
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Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling
Amplification of difficult templates for successful DNA sequencing
18 h
illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.
illustra TempliPhi Sequence Resolver Kit