Invitrogen™ Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with Tempus™ Blood RNA Tubes

Description

• The Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR makes it possible to perform expression analysis directly from a 500 μL aliquot of human whole blood collected in Tempus Blood RNA tubes without conventional RNA purification
• This simple procedure creates a lysate that can go directly into reverse transcription and real-time PCR with the sensitivity and specificity equivalent to conventionally purified RNA
• This kit saves time, generates much less waste, and offers a simple workflow that is suitable for a few samples or can easily be incorporated into automated, high throughput applications
• • Detect total RNA (including miRNA) from Tempus™ Blood RNA Tubes without purification • Robust—500μL sample processed at room temperature with results equivalent to those from purified RNA • Flexible Format—Samples can be prepared in microfuge tubes or manual/automated 96-well plates Simple Workflow Without Sacrificing Sensitivity & Specificity The process consists of pelleting and washing a 500uL aliquot from the blood tube, followed by a unique digestion/DNase step which preserves RNA integrity
• The preparation takes place entirely at room temperature unlike other products that require temperature-specific incubation steps
• Up to 96 samples can be prepared in half the time it takes for competitive spin column purification to process only 12 samples, with less hands-on effort
• The resulting lysate can be stored for later use (1 hour at 4°C or 5 months frozen)
• This straightforward procedure takes place in the same sample processing well, from start to finish, meaning no sample transfers
• The real-time RT-PCR data generated from the lysate is equivalent to results obtained from conventionally purified RNA from the same tubes and has been validated with the following qPCR reagents: • Applied Biosystems™ High Capacity RNA-to-cDNA™ Kit and TaqMan™ Gene Expression Master Mix • Invitrogen's SuperScript™ VILO™ cDNA Synthesis Kit and TaqMan Gene Expression Master Mix • Applied Biosystems™ TaqMan MicroRNA Reverse Transcription Kit and TaqMan Universal Master Mix II • Applied Biosystems™ TaqMan™ One-Step RT-PCR Master Mix Reagents Kit Reverse transcription reagents for cDNA synthesis, TaqMan master mixes, and TaqMan assays for real-time PCR analysis are sold separately and are not included with the kit
• The total number of reverse transcription and PCR reactions possible from one starting sample depends on the minimum sample requirements of the desired RT-PCR kits (Refer to user documentation for details)
• Screen, Discover, or Validate For users with specific genes of interest, the Stabilized Blood-to-C T ™ Nucleic Acid Preparation Kit for qPCR was designed to enable quick screening from a small amount of starting sample (approximately 5% of the total volume of a properly filled Tempus Blood RNA Tube per sample)
• The remainder of the blood tube can be archived for later use or purified as needed
• Those who perform discovery or validation experiments with real-time RT-PCR analysis will save effort, time, and overall cost with the Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR – while employing a scalable process allowing labs bottlenecked by conventional purification to finally start processing samples with ease
• miRNA Analysis With the growing focus on non-coding RNA and its regulatory role in development and disease, it's reassuring to know these small but important RNA molecules can be detected properly with the Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR
• Our scientists have tested the lysate with the Applied Biosystems TaqMan MicroRNA Reverse Transcription Kit and TaqMan Universal Master Mix II against a large panel of TaqMan™ microRNA assays and generated results equal to or better than those of the leading competitor's miRNA purification product
• Reduced Footprint & Less Waste = More Savings The Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR simplifies workflow and reduces total plastic waste by 75% (by weight) for a comparable number of samples processed via columns and generates no organic waste
• Space-saving footprint maximizes freezer capacity
• For Users Needing Full Purification If conventional RNA purification is still required for the remnant blood tube, use the MagMAX for Stabilized Blood Tubes RNA Isolation Kit
• This kit maximizes yield of total RNA from Tempus Blood RNA Tubes using magnetic bead-based separation technology
• Blood tubes, reverse transcription reagents for cDNA synthesis, and TaqMan™ master mixes for real-time PCR analysis sold separately.