Invitrogen™ ChargeSwitch™ gDNA Rendered Meat Purification Kit

Description

• The ChargeSwitch™ gDNA Rendered Meat Purification Kit allows for rapid and efficient purification of genomic DNA (gDNA) from animal feed, animal meal, and heparin products for downstream molecular analysis
• The kit is listed in the U.S
• Food and Drug Administration Center for Veterinary Medicine's protocols as a sample preparation method suitable for monitoring crude heparin quality and testing animal feed for banned materials such as specific animal proteins
• Note: The number of reactions possible with this kit is dependent upon the starting sample type: animal feed and meal samples = 50 preparations, heparin products = 25 preparations
• The ChargeSwitch™ gDNA Rendered Meat Purification Kit protocol starts with prepared lysates and takes approximately 15 minutes to complete without centrifugation, vacuum, or organic solvents (for batches of up to 5 samples per operator—larger batch sizes will require longer processing times)
• The kit employs a magnetic bead with a switchable surface
• Low pH conditions cause binding of DNA to the bead while high pH conditions cause the release of the DNA
• Tested by the U.S
• Food and Drug Administration The ChargeSwitch™ gDNA Rendered Meat Purification Kit has been thoroughly tested by the U.S
• Food and Drug Administration for processing up to 500 mg of animal feed, 250 mg of animal meal, and 250 mg of heparin product per reaction
• References Development of a Multiplex Real-Time PCR Assay for the Detection of Ruminant DNA in Raw Materials used for Monitoring Crude Heparin for Quality: www.fda.gov/AnimalVeterinary/ScienceResearch/ToolsResources/ucm350289.html Development, evaluation, and peer verification of a rapid real-time PCR method for the detection of animal material
• Journal of Food Protection, Vol
• 72, No
• 11, 2009, Pages 2368–2374
• U.S
• Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, Maryland 20708, USA.