45-001-498

Cytiva plasmidPrep Midi Flow Kit

Manufacturer: Cytiva

The price for this product is unavailable. Please request a quote

Description

illustra plasmidPrep Midi Flow Kit is designed for the purification of high yields of transfection-grade plasmid DNA. The kit allows for the purification of large yields of plasmid DNA that are proportional to the input sample volume.

Includes

Fast-flow genomic 250 columns, lyophilized Proteinase K powder, Lysis solutions, load and Elution Buffers, NAP-25 desalting column, spin column adapters, full protocol booklet, detachable, quick reference protocol card

Product Type

illustra plasmidPrep Midi Flow Kit

Sample Volume

150 mL Culture

For Use With (Application)

Used For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification

No. of Reactions

25 Preps

Purity

1.8

Test Time

Approx.215 min

Related Products

Img

Invitrogen™

K210001

--

Img

Invitrogen™

CS31104

--

Img

Invitrogen™

K191001

--

Img

Invitrogen™

K190001

--

Img

Cytiva

45-001-488

--

Img

Invitrogen™

K210009XP

--

Img

Invitrogen™

CS31103

--

Img

Invitrogen™

K210008XP

--

Description

  • The kit consists of a high-performance, anion-exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA
  • High column capacity allows for a proportional yield for input sample volume of 25 to 50mL (for high copy number plasmid) and up to 500mL for low copy number plasmid Provides plasmid DNA with excellent purity and low endotoxin levels Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns

Compare Similar Items

Show Difference

Img

Cytiva

45-001-498

--


Description:
illustra plasmidPrep Midi Flow Kit is designed for the purification of high yields of transfection-grade plasmid DNA. The kit allows for the purification of large yields of plasmid DNA that are proportional to the input sample volume.

Includes:
Fast-flow genomic 250 columns, lyophilized Proteinase K powder, Lysis solutions, load and Elution Buffers, NAP-25 desalting column, spin column adapters, full protocol booklet, detachable, quick reference protocol card

Product Type:
illustra plasmidPrep Midi Flow Kit

Sample Volume:
150 mL Culture

For Use With (Application):
Used For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification

No. of Reactions:
25 Preps

Purity:
1.8

Test Time:
Approx.215 min

Img

Fischer Scientific

45-001-499

--


Description:
Purify high yields of plasmid DNA in less than 10 min. Color-coded tubes and columns simplify the protocol and purified DNA is suitable for all common downstream applications.

Includes:
Spin columns prepacked with a novel Silica membrane, Suspension, Lysis and Neutralization Buffers containing chaotropic salts, Wash and Elution Buffers, microcentrifuge collection tubes, full protocol booklet, Detachable, quick reference protocol card

Product Type:
illustra plasmidPrep Mini Spin Kit

Sample Volume:
3.0 mL

For Use With (Application):
Suitable for many downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCRSequencing, PCR, ligation and cloning, restriction digest, enzymatic modification, template for labeled probe generation, transforma

No. of Reactions:
50 Preps

Purity:
1.7

Test Time:
Approx. 9 min

Img

Fischer Scientific

45-001-500

--


Description:
Purify high yields of plasmid DNA in less than 10 min. Color-coded tubes and columns simplify the protocol and purified DNA is suitable for all common downstream applications.

Includes:
Spin columns prepacked with a novel Silica membrane, Suspension, Lysis and Neutralization Buffers containing chaotropic salts, Wash and Elution Buffers, microcentrifuge collection tubes, full protocol booklet, Detachable, quick reference protocol card

Product Type:
illustra plasmidPrep Mini Spin Kit

Sample Volume:
1.5 mL

For Use With (Application):
Suitable for many downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCRSequencing, PCR, ligation and cloning, restriction digest, enzymatic modification, template for labeled probe generation, transforma

No. of Reactions:
250 Preps

Purity:
1.7

Test Time:
Approx. 9 min

Img

Fischer Scientific

45-001-502

--


Description:
illustra tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from a variety of animal tissues and mammalian cell cultures in just 90 min for tissues and 45 min for ce

Includes:
Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card

Product Type:
illustra tissue and cells genomicPrep Mini Spin Kit

Sample Volume:
5-50 mg

For Use With (Application):
Used for several downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR

No. of Reactions:
50 Preps

Purity:
1.75

Test Time:
90 min[Animal Tissue], 45 min[Cultured cells]