Cytiva illustra AutoSeq G-50

Description

• illustra AutoSeq G-50 columns are specifically designed to remove residual fluorescent dye-terminators that can obscure data in automated sequencing
• illustra AutoSeq G-50 consists of MicroSpin columns containing Sephadex G-50, pre-equilibrated in double-distilled water
• This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers
• For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated sequencing Columns are prepacked with Sephadex G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative Columns that are ready to use enable sample application to collection of purified product in less than 4 minutes Can also be used to clean up reactions prepared with the BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems.