MP Biomedicals™ Gnome™ DNA Isolation Kit
Gnome™ DNA Isolation Kit offers a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types.
Manufacturer: Fischer Scientific
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Format
Kit
Includes
Cell Lysis/Denaturing Solution, RNase Mixx, Protease Mixx, Salt Out Mixture, Cell Suspension Solution
Product Type
Gnome™ DNA Isolation Kit
Yield
100 μg
Sample Type
Blood, Tissues, Yeast, Bacteria, Plant
pH
Cell Lysis/Denaturing Solution pH range: 6.2 to 6.9, Salt-out Mixture pH range: 6.0 to 6.8, Cell Suspension Solution pH range: 8.0 to 8.2
Sample Volume
20 to 50 mg wet weight bacterial cell pellet, Up to 250 mg plant tissue, 2 to 5 x 107 nucleated white blood cells, 50 to 250 mg mammalian tissue, ∼1 to 5 x 108 yeast cell pellet
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Description
- No organic extractions are required, and up to 100 μg of DNA can be isolated per prep
- The GNOME™ kit utilizes RNase Mixx to eliminate RNA immediately after lysis, and Protease Mixx to rapidly degrade cellular proteins
- This is followed by a proprietary ''salting-out'' technique which precludes the need for phenol, chloroform, or other organic extractions
- The DNA can either be spooled, removed with forceps, or centrifuged and dissolved in TE
- The GNOME™ DNA Isolation kit is used to quickly and efficiently isolate high molecular weight genomic DNA from cells and tissues of any type, including bacteria, yeast, plant, and animal cells
- Each preparation with the GNOME™ kit yields up to 100 μg of genomic DNA
- The DNA isolated by the GNOME™ procedure is suitable for restriction enzyme digestion or PCR amplification in as little as 1 hour after cell lysis.
Compare Similar Items
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Format: Kit
Includes: Cell Lysis/Denaturing Solution, RNase Mixx, Protease Mixx, Salt Out Mixture, Cell Suspension Solution
Product Type: Gnome™ DNA Isolation Kit
Yield: 100 μg
Sample Type: Blood, Tissues, Yeast, Bacteria, Plant
pH: Cell Lysis/Denaturing Solution pH range: 6.2 to 6.9, Salt-out Mixture pH range: 6.0 to 6.8, Cell Suspension Solution pH range: 8.0 to 8.2
Sample Volume: 20 to 50 mg wet weight bacterial cell pellet, Up to 250 mg plant tissue, 2 to 5 x 107 nucleated white blood cells, 50 to 250 mg mammalian tissue, ∼1 to 5 x 108 yeast cell pellet
Format: __
Includes: __
Product Type: SPIN Module and Recovery Tubes
Yield: __
Sample Type: Lysate or other suspension
pH: __
Sample Volume: 500 to 700 μL liquid per centrifugation, Larger volumes require multiple loadings
Format: __
Includes: __
Product Type: SPIN Module and Recovery Tubes
Yield: __
Sample Type: Lysate or other suspension
pH: __
Sample Volume: 500 to 700 μL liquid per centrifugation, Larger volumes require multiple loadings
