Invitrogen™ Chargeswitch™ Pro Filter Plasmid Maxi Kit

Description

• The Chargeswitch™ -Pro Filter Plasmid Maxi Kit includes 10 plasmid maxipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol
• The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications
• The system is based upon a novel dual/nested column design which facilitate the fastest and simplest protocol for high quality plasmid
• The system is composed of two columns
• The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate
• The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate
• This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step
• The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience
• To accomplish this, the ChargeSwitch™-Pro Filter Kit provides: • 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method • Cleaner purified product for improved performance in downstream applications • A novel Dual/nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol • Endotoxin units less than 10 EU/ug DNA for • Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (Cat
• No
• K211101) How it works The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism
• At low pH values (less than 6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA
• Raising the pH (greater than 8.5) neutralizes the charge and elutes the bound nucleic acid
• The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless.