Thermo Scientific™ MagMAX™ Plant DNA Isolation Kit

Description

• The MagMAX™ Plant DNA Isolation Kit is designed for automated high-throughput or manual purification of DNA from a wide variety of plant species
• The kit uses MagMAX magnetic bead technology, eliminating the need for phenol/chloroform extraction or alcohol precipitation
• The procedures are optimized for the isolation of DNA from 10–100 mg plant samples
• DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, age of tissue sample, and storage conditions
• The MagMAX Plant DNA Isolation Kit relies on magnetic bead technology, enabling reproducible yields and robust performance
• High binding capacity, uniform particle size, and rapid magnetic response of the beads makes the technology ideal for automated high-throughput nucleic acid purification, as well as for manual purification for low sample throughput
• The resulting high-quality DNA is free of proteins, nucleases, and other contaminants or inhibitors, and can be used in a wide range of downstream applications such as PCR, qPCR, or next-generation sequencing
• See bar graph below for typical genomic DNA yields from various sources
• Features of the MagMAX Plant DNA Isolation Kit include: • Versatile—suitable for most plant types and parts, including woody, polyphenol-rich, and lignified samples • High DNA yields—compared to traditional CTAB method • High quality—isolates high integrity DNA, free of inhibitors and RNA contamination • Flexible—easily adjustable for high-throughput automation platforms and manual processing Applications Isolated DNA is ideal for use in common molecular biology procedures, including: • qPCR • next-generation sequencing Benefits of MagMAX magnetic bead–based purification The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher™ Duo Prime or KingFisher™ Flex processors
• Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids
• Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields
• Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process
• This clogging issue is of particular concern with fibrous plant tissue samples that that are not fully lysed during the disruption and homogenization steps
• Optimized bead surface chemistry The Dynabeads™ MyOne™ Carboxylic Acid utilized in the MagMAX Plant DNA Kit has an optimized surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture
• These aspects make Dynabeads magnetic technology ideal for the recovery of the DNA targets found in a variety of plant samples
• Once captured, the DNA is then eluted in 150 μL and ready for downstream applications.