A35852
Applied Biosystems™ MicroSEQ™ ID Purification Combo Kit v2.0, with clean-up plates
Manufacturer: Applied Biosystems™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A35852-Each-of-1 | In Stock | ₹ 50,641.00 |
A35852 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,641.00
GST (18%): ₹ 9,115.38
Total Price: ₹ 59,756.38
Content And Storage
• 2 x ExoSAP-IT Express Reagent (100 reactions). Store at -5°C to -30°C.• 2 x MicroSEQ ID Ultra Sequencing Clean-up Plate (96-well). Store at 2°C to 8°C.
Sample Type
Bacteria, DNA, dsDNA, Fungi
High-throughput Compatibility
High-throughput Compatible, Manual Protocols
Product Line
MicroSEQ™
Target
Fluorescent Dye Removal, DNA from Enzymatic Reactions (e.g. PCR)
Format
Plate
For Use With (Application)
Sequencing, PCR
No. of Reactions
100 Reactions
Shipping Condition
Approved for shipment on Wet or Dry Ice
Related Products
Description
- The MicroSEQ ID Purification Combo Kit v2.0 includes reagents for post-PCR and post-sequencing clean-up in the MicroSEQ ID microbial identification workflow
- This version contains one tube of ExoSAP-IT Express Reagent for post-PCR clean-up and two 96- well MicroSEQ ID Ultra Sequencing Clean-up Plates for post-sequencing clean-up
- ExoSAP-IT Express Reagent removes unincorporated dNTPs and primers from the post-amplification PCR mix in 5 minutes compared to 30 minutes with the original ExoSAP-IT Reagent while producing equivalent results
- The MicroSEQ ID Ultra Sequencing Clean-up kit in 96-well plate or 8-strips format provides a convenient and simple way to remove dye terminators, dNTPs, salts, and residual primers from cycle sequencing reactions
- The end products can be used directly for sequencing on any Applied Biosystems genetic analyzer
- Comparability studies demonstrated equivalent results between the original ExoSAP-IT Reagent and the improved ExoSAP-IT Express Reagent coupled with the MicroSEQ ID Ultra Sequencing Clean-Up kit in the MicroSEQ ID workflow (see figure below)
- Both ExoSAP-IT and ExoSAP-IT Express enzyme-treated PCR samples produced very similar sequencing results and the correct identification result
- ExoSAP-IT Express Reagent can be used downstream of the following PCR amplification kits: MicroSEQ 500 16S rDNA PCR Kit MicroSEQ Full Gene 16S rDNA PCR Kit MicroSEQD2 rDNA Fungal PCR Kit MicroSEQ ID Ultra Sequencing Clean-up Plates can be used downstream of the following sequencing kits: MicroSEQ 500 16S rDNA Sequencing Kit MicroSEQ Full Gene 16S rDNA Sequencing Kit MicroSEQ D2 rDNA Fungal Sequencing Kit
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Content And Storage: • 2 x ExoSAP-IT Express Reagent (100 reactions). Store at -5°C to -30°C.• 2 x MicroSEQ ID Ultra Sequencing Clean-up Plate (96-well). Store at 2°C to 8°C.
Sample Type: Bacteria, DNA, dsDNA, Fungi
High-throughput Compatibility: High-throughput Compatible, Manual Protocols
Product Line: MicroSEQ™
Target: Fluorescent Dye Removal, DNA from Enzymatic Reactions (e.g. PCR)
Format: Plate
For Use With (Application): Sequencing, PCR
No. of Reactions: 100 Reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice
Content And Storage:
• 2 x ExoSAP-IT Express Reagent (100 reactions). Store at -5°C to -30°C.• 2 x MicroSEQ ID Ultra Sequencing Clean-up Plate (96-well). Store at 2°C to 8°C.
Sample Type:
Bacteria, DNA, dsDNA, Fungi
High-throughput Compatibility:
High-throughput Compatible, Manual Protocols
Product Line:
MicroSEQ™
Target:
Fluorescent Dye Removal, DNA from Enzymatic Reactions (e.g. PCR)
Format:
Plate
For Use With (Application):
Sequencing, PCR
No. of Reactions:
100 Reactions
Shipping Condition:
Approved for shipment on Wet or Dry Ice
Content And Storage: • 1 x ExoSAP-IT Express Reagent (100 reactions). Store at -5°C to -30°C.• 1 x MicroSEQ ID Ultra Sequencing Clean-up 8-Strips Kit (12 x 8 strips). Store at 2°C to 8°C.
Sample Type: Bacteria, DNA, dsDNA, Fungi
High-throughput Compatibility: High-throughput Compatible, Manual Protocols
Product Line: MicroSEQ™
Target: Fluorescent Dye Removal, DNA from Enzymatic Reactions (e.g. PCR)
Format: Strip
For Use With (Application): Sequencing, PCR
No. of Reactions: 100 Reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice
Content And Storage:
• 1 x ExoSAP-IT Express Reagent (100 reactions). Store at -5°C to -30°C.• 1 x MicroSEQ ID Ultra Sequencing Clean-up 8-Strips Kit (12 x 8 strips). Store at 2°C to 8°C.
Sample Type:
Bacteria, DNA, dsDNA, Fungi
High-throughput Compatibility:
High-throughput Compatible, Manual Protocols
Product Line:
MicroSEQ™
Target:
Fluorescent Dye Removal, DNA from Enzymatic Reactions (e.g. PCR)
Format:
Strip
For Use With (Application):
Sequencing, PCR
No. of Reactions:
100 Reactions
Shipping Condition:
Approved for shipment on Wet or Dry Ice
Content And Storage: • Resuspension buffer, store at 4°C upon reciept• Lysis buffer, store at room temperature• Precipitation buffer, store at room temperature• Binding buffer, store at room temperature• Wash buffer 1, store at room temperature• Wash buffer 2 (concentrate), store at room temperature• Elution buffer, store at room temperature• Column assemblies, store at room temperature• Syringe filters, store at room temperature• Syringe plungers, store at room temperature• Collection tubes, store at room temperature
Sample Type: __
High-throughput Compatibility: __
Product Line: PureLink™
Target: __
Format: Kit
For Use With (Application): Cloning, PCR
No. of Reactions: 25 Preps
Shipping Condition: Room Temperature
Content And Storage:
• Resuspension buffer, store at 4°C upon reciept• Lysis buffer, store at room temperature• Precipitation buffer, store at room temperature• Binding buffer, store at room temperature• Wash buffer 1, store at room temperature• Wash buffer 2 (concentrate), store at room temperature• Elution buffer, store at room temperature• Column assemblies, store at room temperature• Syringe filters, store at room temperature• Syringe plungers, store at room temperature• Collection tubes, store at room temperature
Sample Type:
__
High-throughput Compatibility:
__
Product Line:
PureLink™
Target:
__
Format:
Kit
For Use With (Application):
Cloning, PCR
No. of Reactions:
25 Preps
Shipping Condition:
Room Temperature
Content And Storage: • Resuspension buffer, store at 4°C upon reciept• Lysis buffer, store at room temperature• Precipitation buffer, store at room temperature• Binding buffer, store at room temperature• Wash buffer 1, store at room temperature• Wash buffer 2 (concentrate), store at room temperature• Elution buffer, store at room temperature• Column assemblies, store at room temperature• Syringe filters, store at room temperature• Syringe plungers, store at room temperature• Collection tubes, store at room temperature
Sample Type: __
High-throughput Compatibility: __
Product Line: PureLink™
Target: __
Format: Kit
For Use With (Application): Cloning, PCR
No. of Reactions: 10 Preps
Shipping Condition: Room Temperature
Content And Storage:
• Resuspension buffer, store at 4°C upon reciept• Lysis buffer, store at room temperature• Precipitation buffer, store at room temperature• Binding buffer, store at room temperature• Wash buffer 1, store at room temperature• Wash buffer 2 (concentrate), store at room temperature• Elution buffer, store at room temperature• Column assemblies, store at room temperature• Syringe filters, store at room temperature• Syringe plungers, store at room temperature• Collection tubes, store at room temperature
Sample Type:
__
High-throughput Compatibility:
__
Product Line:
PureLink™
Target:
__
Format:
Kit
For Use With (Application):
Cloning, PCR
No. of Reactions:
10 Preps
Shipping Condition:
Room Temperature