Applied Biosystems™ MicroSEQ™ ID Purification Combo Kit v2.0, with cartridges

Description

• The MicroSEQ ID Purification Combo Kit v2.0 includes reagents for post-PCR and post-sequencing clean-up in the MicroSEQ ID microbial identification workflow
• This version contains one tube of ExoSAP-IT Express Reagent for post-PCR clean-up and MicroSEQ ID Sequencing Clean-up Cartridges for post-sequencing clean-up
• ExoSAP-IT Express Reagent removes unincorporated dNTPs and primers from the post-amplification PCR mix in 5 minutes compared to 30 minutes with the original ExoSAP-IT Reagent while producing equivalent results
• The MicroSEQ ID Sequencing Clean-up Cartridges provide a convenient and simple way to remove dye terminators, dNTPs, salts, and residual primers from cycle sequencing reactions
• The end products can be used directly for sequencing on any Applied Biosystems genetic analyzer
• Comparability studies demonstrated equivalent results between the original ExoSAP-IT Reagent and the improved ExoSAP-IT Express Reagent coupled with the MicroSEQ ID Sequencing Clean-Up kit in the MicroSEQ ID workflow (see figure 1)
• Both ExoSAP-IT and ExoSAP-IT Express enzyme-treated PCR samples produced very similar sequencing results and the correct identification result
• ExoSAP-IT Express Reagent can be used downstream of the following PCR amplification kits: MicroSEQ 500 16S rDNA PCR Kit MicroSEQ Full Gene 16S rDNA PCR Kit MicroSEQD2 rDNA Fungal PCR Kit MicroSEQ ID Sequencing Clean-up Cartridges can be used downstream of the following sequencing kits: MicroSEQ 500 16S rDNA Sequencing Kit MicroSEQ Full Gene 16S rDNA Sequencing Kit MicroSEQ D2 rDNA Fungal Sequencing Kit