CS1102010

Invitrogen™ ChargeSwitch™ gDNA Normalized Buccal Cell Kit

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 CS1102010-Each-of-1 In Stock ₹ 3,84,560.00

CS1102010 - Each of 1

₹ 3,84,560.00

In Stock

Quantity

1

Base Price: ₹ 3,84,560.00

GST (18%): ₹ 69,220.80

Total Price: ₹ 4,53,780.80

Cell Type

Buccal Cell

Content And Storage

Store in refrigerator (2–8°C).

Sample Type

Buccal Samples

High-throughput Compatibility

High-throughput Compatible

Product Line

ChargeSwitch™

Target

Genomic DNA

Concentration

1 ng/μL to 3 ng/μL

Isolation Technology

ChargeSwitch Chemistry

For Use With (Application)

Forensic Testing, PCR

No. of Reactions

960 Preps

Shipping Condition

Room Temperature

Test Time

15 min.

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Description

  • Description The ChargeSwitch™ gDNA Normalized Buccal Cell Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from human buccal swabs in less than 15 minutes after lysate preparation
  • • Optimized for higher-purity DNA extraction • Ideal for STR profiling • Compatible with high-throughput protocols Rapid Purification Protocol The ChargeSwitch™ gDNA Normalized Buccal Cell Kit allows purification of genomic DNA from human buccal cell swabs or pelleted cells from a mouthwash in less than 15 minutes after lysates preparation
  • ChargeSwitch™ gDNA Normalized Buccal Cell Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification
  • High-quality genomic DNA is purified at a normalized concentration of 1-3 ng/μL in a total volume of 150 μL (Figure 1) by eliminating the need for lengthy quantitation and dilution steps
  • Samples can be stored for up to 2 weeks at 4°C before processing without a noticeable loss in DNA yield or quality
  • Note: DNA yield varies and is dependent on several factors including the technique of the person taking the swab, whether the donor is a high or low shedder, and the type of swab used
  • Validated for Single Tandem Repeat (STR) Profiling After employing the ChargeSwitch™ gDNA Normalized Buccal Cell Kit, purified gDNA is suitable for use in STR analysis of poor or environmentally degraded samples, and for downstream applications such as PCR
  • Compatible with Single Tube and High-Throughput Protocols The ChargeSwitch™ gDNA Normalized Buccal Cell Kit is designed for automated processing of both small numbers of samples individually, as well as large numbers of samples using a liquid handling robot or a multi-channel pipette
  • For processing up to 24 individual samples at a time the MagnaRack™ (Cat
  • No
  • CS15000) two-piece magnetic separation tube rack is required
  • This rack consists of a magnetic base station and a removable tube rack, which can hold up to 24 microcentrifuge tubes and can be used with most ChargeSwitch™ products
  • For high-throughput applications utilizing 96 well blocks, the 96-well Magnetic Separator (Cat
  • No
  • CS15096) is required
  • This rack can hold and separate up to 96 magnetic bead samples in a deep well plate when placed on top of the magnetic base station
  • For Research Use Only
  • Not intended for diagnostic procedures
  • Order Info Shipping Condition: Room Temperature

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Fischer Scientific

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Cell Type:
Buccal Cell

Content And Storage:
Store in refrigerator (2–8°C).

Sample Type:
Buccal Samples

High-throughput Compatibility:
High-throughput Compatible

Product Line:
ChargeSwitch™

Target:
Genomic DNA

Concentration:
1 ng/μL to 3 ng/μL

Isolation Technology:
ChargeSwitch Chemistry

For Use With (Application):
Forensic Testing, PCR

No. of Reactions:
960 Preps

Shipping Condition:
Room Temperature

Test Time:
15 min.

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Content And Storage:
All components of the ChargeSwitch™ Forensic DNA Purification Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.Components:• ChargeSwitch™ Lysis Buffer (L13)• ChargeSwitch™ Magnetic Beads• Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)• ChargeSwitch™ Purification Buffer (N5)• ChargeSwitch™ Wash Buffer (W12)• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

Sample Type:
Forensic Sample

High-throughput Compatibility:
High-throughput Compatible

Product Line:
ChargeSwitch™

Target:
Genomic DNA

Concentration:
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Cell Type:
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Content And Storage:
All components of the ChargeSwitch™ Forensic DNA Purification Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.Components:• ChargeSwitch™ Lysis Buffer (L13)• ChargeSwitch™ Magnetic Beads• Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)• ChargeSwitch™ Purification Buffer (N5)• ChargeSwitch™ Wash Buffer (W12)• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

Sample Type:
Forensic Sample

High-throughput Compatibility:
High-throughput Compatible

Product Line:
ChargeSwitch™

Target:
Genomic DNA

Concentration:
__

Isolation Technology:
ChargeSwitch Chemistry

For Use With (Application):
Forensic

No. of Reactions:
960 Preps

Shipping Condition:
Room Temperature

Test Time:
__

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Cell Type:
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Content And Storage:
All components of the ChargeSwitch™ Forensic DNA Purification Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.

Sample Type:
__

High-throughput Compatibility:
__

Product Line:
ChargeSwitch™

Target:
__

Concentration:
__

Isolation Technology:
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For Use With (Application):
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No. of Reactions:
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Shipping Condition:
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