4926406
Invitrogen™ ChargeSwitch™ gDNA Normalized Buccal Cell Kit
The ChargeSwitch™ gDNA Normalized Buccal Cell Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from human buccal swabs in less than 15 minutes after lysate preparation.
Manufacturer: Fischer Scientific
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Cell Type
Buccal Cell
Content And Storage
Store in refrigerator (2–8°C).
Sample Type
Buccal Samples
Product Line
ChargeSwitch™
Target
Genomic DNA
Concentration
1 ng/μL to 3 ng/μL
Isolation Technology
ChargeSwitch Chemistry
High-throughput Compatibility
High-throughput Compatible
Shipping Condition
Room Temperature
Test Time
15 min.
Related Products
Description
- The ChargeSwitch™ gDNA Normalized Buccal Cell Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from human buccal swabs in less than 15 minutes after lysate preparation
- • Optimized for higher-purity DNA extraction • Ideal for STR profiling • Compatible with high-throughput protocols Rapid Purification Protocol The ChargeSwitch™ gDNA Normalized Buccal Cell Kit allows purification of genomic DNA from human buccal cell swabs or pelleted cells from a mouthwash in less than 15 minutes after lysates preparation
- ChargeSwitch™ gDNA Normalized Buccal Cell Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification
- High-quality genomic DNA is purified at a normalized concentration of 1-3 ng/μL in a total volume of 150 μL (Figure 1) by eliminating the need for lengthy quantitation and dilution steps
- Samples can be stored for up to 2 weeks at 4°C before processing without a noticeable loss in DNA yield or quality
- Note: DNA yield varies and is dependent on several factors including the technique of the person taking the swab, whether the donor is a high or low shedder, and the type of swab used
- Validated for Single Tandem Repeat (STR) Profiling After employing the ChargeSwitch™ gDNA Normalized Buccal Cell Kit, purified gDNA is suitable for use in STR analysis of poor or environmentally degraded samples, and for downstream applications such as PCR
- Compatible with Single Tube and High-Throughput Protocols The ChargeSwitch™ gDNA Normalized Buccal Cell Kit is designed for automated processing of both small numbers of samples individually, as well as large numbers of samples using a liquid handling robot or a multi-channel pipette
- For processing up to 24 individual samples at a time the MagnaRack™ (Cat
- No
- CS15000) two-piece magnetic separation tube rack is required
- This rack consists of a magnetic base station and a removable tube rack, which can hold up to 24 microcentrifuge tubes and can be used with most ChargeSwitch™ products
- For high-throughput applications utilizing 96 well blocks, the 96-well Magnetic Separator (Cat
- No
- CS15096) is required
- This rack can hold and separate up to 96 magnetic bead samples in a deep well plate when placed on top of the magnetic base station
- For Research Use Only
- Not intended for diagnostic procedures
- Order Info Shipping Condition: Room Temperature
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Cell Type: Buccal Cell
Content And Storage: Store in refrigerator (2–8°C).
Sample Type: Buccal Samples
Product Line: ChargeSwitch™
Target: Genomic DNA
Concentration: 1 ng/μL to 3 ng/μL
Isolation Technology: ChargeSwitch Chemistry
High-throughput Compatibility: High-throughput Compatible
Shipping Condition: Room Temperature
Test Time: 15 min.
Cell Type:
Buccal Cell
Content And Storage:
Store in refrigerator (2–8°C).
Sample Type:
Buccal Samples
Product Line:
ChargeSwitch™
Target:
Genomic DNA
Concentration:
1 ng/μL to 3 ng/μL
Isolation Technology:
ChargeSwitch Chemistry
High-throughput Compatibility:
High-throughput Compatible
Shipping Condition:
Room Temperature
Test Time:
15 min.
Cell Type: __
Content And Storage: All components of the ChargeSwitch™ gDNA Plant Kit are shipped at room temperature. Upon receipt, store RNase A at 4°C. Store all other kit components at room temperature.Components:• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)• ChargeSwitch™ Lysis Buffer (L18)• ChargeSwitch™ Precipitation Buffer (N5)• ChargeSwitch™ Wash Buffer (W12)• ChargeSwitch™ Elution Buffer (E6; 10 mM Tris-HCl, pH 8.5 with 1 mM EDTA)• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)• 10% SDS in deionized water• 10% Detergent (D1)
Sample Type: __
Product Line: ChargeSwitch™
Target: Genomic DNA
Concentration: __
Isolation Technology: ChargeSwitch Chemistry
High-throughput Compatibility: High-throughput Compatible
Shipping Condition: Room Temperature
Test Time: 15 min.
Cell Type:
__
Content And Storage:
All components of the ChargeSwitch™ gDNA Plant Kit are shipped at room temperature. Upon receipt, store RNase A at 4°C. Store all other kit components at room temperature.Components:• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)• ChargeSwitch™ Lysis Buffer (L18)• ChargeSwitch™ Precipitation Buffer (N5)• ChargeSwitch™ Wash Buffer (W12)• ChargeSwitch™ Elution Buffer (E6; 10 mM Tris-HCl, pH 8.5 with 1 mM EDTA)• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)• 10% SDS in deionized water• 10% Detergent (D1)
Sample Type:
__
Product Line:
ChargeSwitch™
Target:
Genomic DNA
Concentration:
__
Isolation Technology:
ChargeSwitch Chemistry
High-throughput Compatibility:
High-throughput Compatible
Shipping Condition:
Room Temperature
Test Time:
15 min.
Cell Type: __
Content And Storage: All components of the ChargeSwitch™ gDNA Plant Kit are shipped at room temperature. Upon receipt, store RNase A at 4°C. Store all other kit components at room temperature.Components:• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)• ChargeSwitch™ Lysis Buffer (L18)• ChargeSwitch™ Precipitation Buffer (N5)• ChargeSwitch™ Wash Buffer (W12)• ChargeSwitch™ Elution Buffer (E6; 10 mM Tris-HCl, pH 8.5 with 1 mM EDTA)• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)• 10% SDS in deionized water• 10% Detergent (D1)
Sample Type: __
Product Line: ChargeSwitch™
Target: Genomic DNA
Concentration: __
Isolation Technology: ChargeSwitch Chemistry
High-throughput Compatibility: High-throughput Compatible
Shipping Condition: Room Temperature
Test Time: 15 min.
Cell Type:
__
Content And Storage:
All components of the ChargeSwitch™ gDNA Plant Kit are shipped at room temperature. Upon receipt, store RNase A at 4°C. Store all other kit components at room temperature.Components:• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)• ChargeSwitch™ Lysis Buffer (L18)• ChargeSwitch™ Precipitation Buffer (N5)• ChargeSwitch™ Wash Buffer (W12)• ChargeSwitch™ Elution Buffer (E6; 10 mM Tris-HCl, pH 8.5 with 1 mM EDTA)• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)• 10% SDS in deionized water• 10% Detergent (D1)
Sample Type:
__
Product Line:
ChargeSwitch™
Target:
Genomic DNA
Concentration:
__
Isolation Technology:
ChargeSwitch Chemistry
High-throughput Compatibility:
High-throughput Compatible
Shipping Condition:
Room Temperature
Test Time:
15 min.
Cell Type: __
Content And Storage: The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly.Components:• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)• ChargeSwitch™ Purification Buffer (N5)• ChargeSwitch™ Wash Buffer (W12)• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Sample Type: PCR Products
Product Line: ChargeSwitch™
Target: DNA from Enzymatic Reactions (e.g. PCR)
Concentration: __
Isolation Technology: ChargeSwitch™ Chemistry
High-throughput Compatibility: High-throughput Compatible
Shipping Condition: Room Temperature
Test Time: __
Cell Type:
__
Content And Storage:
The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly.Components:• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)• ChargeSwitch™ Purification Buffer (N5)• ChargeSwitch™ Wash Buffer (W12)• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Sample Type:
PCR Products
Product Line:
ChargeSwitch™
Target:
DNA from Enzymatic Reactions (e.g. PCR)
Concentration:
__
Isolation Technology:
ChargeSwitch™ Chemistry
High-throughput Compatibility:
High-throughput Compatible
Shipping Condition:
Room Temperature
Test Time:
__