Thermo Scientific™ GeneJET Plasmid Maxiprep Kit

Description

• Description GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column
• Each prep recovers up to 750 μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines
• Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column
• Each prep recovers up to 750 μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines
• Highlights • Efficient —high yields up to 750 μg of high quality plasmid DNA from 250 mL of bacterial culture • High concentration of DNA purified — >400 ng/μL • Convenient —spin column format, centrifuge or vacuum protocols • Fast —procedure takes 60 minutes, considerably faster than using gravity flow columns Applications Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including: • Transfection of robust cell lines • FastDigest restriction enzyme digestion • Automated fluorescent sequencing • PCR • Cloning • Transformation • In vitro transcription