Thermo Scientific™ GeneJET Endo-Free Plasmid Maxiprep Kit

Description

• The Thermo Scientific GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column
• Each prep recovers up to 750 μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines
• Highlights: Efficient —high yields up to 750 μg of high quality plasmid DNA from 250 mL of bacterial culture High concentration of DNA purified —> 400 ng/μL Convenient —spin column format, centrifuge or vacuum protocols Fast —procedure takes 60 minutes, considerably faster than using gravity flow columns Applications Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including: Transfection of robust cell lines FastDigest restriction enzyme digestion Automated fluorescent sequencing PCR Cloning Transformation In vitro transcription Includes Resuspension Solution Lysis Solution Neutralization Solution Endotoxin Binding Reagent Wash Solution I (concentrated) Wash Solution II (concentrated) RNase A Solution Elution Buffer (10 mM Tris-HCl, pH 8.5) GeneJET Maxi Purification Columns pre-assembled with collection tubes (50 mL) Collection tubes (50 mL) Hazardous : No Quality Control : The GeneJET Plasmid Maxiprep Kit is qualified by isolating high copy number plasmid DNA from 250 mL of overnight E
• coli culture (OD600 = 2) grown in LB medium following the protocol outlined in the manual
• The kit passes QC requirements if the purified plasmid DNA has an A 260/280 ratio between 1.8 and 1.9 and a dominant band of supercoiled plasmid DNA is observed after agarose gel electrophoresis
• The functional quality of the plasmid DNA is evaluated by digestion with restriction endonucleases
• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including: Transfection of robust cell lines FastDigest restriction enzyme digestion Automated fluorescent sequencing PCR Cloning Transformation In vitro transcription Order Info Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.