Thermo Scientific™ Buffers for GeneJET™ngS Cleanup Kit

Description

• The GeneJET NGS Cleanup Kit provides protocols optimized specially for fast DNA fragment cleanup or adapter removal procedures
• The clean up protocol results in higher than 70% recovery of DNA fragments larger than 150 bp and more than 97% removal of adapters
• Adapter removal protocol results in more than 70% recovery of DNA fragments larger than 200 bp and more than 99% removal of adapters
• The entire procedure takes 5 minutes for DNA fragment library cleanup from enzymatic reaction mixtures and 7 minutes for adapter removal protocol
• Spin columns technology in combination with unique binding buffer allows to get the reproducible yields of DNA fragments in the wide starting material range (5 ng - 1 μg of DNA fragments)
• The GeneJET NGS Cleanup Kit is compatible with Illumina™, Life Technologies library preparation workflows
• Highlights Fast - high quality DNA fragments obtained within 5 min
• Robust - reliable cleanup and size selection from different reaction mixtures and buffers, such as end-conversion, ligation, adapter addition, PCR mixtures, fragmentation buffers
• Efficient - DNA recovery up to 90%
• NGS-grade purity - free of primers, adapters, nucleotides, enzymes and other reaction components inhibiting NGS workflow
• Applications Purified DNA fragments are ideal for use in: PCR and qPCR Next Generation Sequencing workflow Includes Binding Buffer Prewash Buffer (conc.) Wash Buffer (conc.) Elution Buffer (10 mM Tris-HCl, pH 8.5) DNA Purification Micro Column & Collection Tube Collection Tube Hazardous: No Quality Control: Thermo Scientific GeneJET NGS Cleanup Kit has been tested by isolating of 300 bp DNA fragment from reaction mixture according the protocol outlined in the manual
• The quality of the purified DNA is evaluated spectrophotometrically and by agarose gel electrophoresis Shelf Life: 36 Months Shipping Condition: Ambient Storage Condition: Ambient