Invitrogen™ S.N.A.P.™ UV-Free Gel Purification Kit

Description

• The S.N.A.P.™ UV-Free Gel Purification Kit uses non-toxic reagents to rapidly purify intact DNA fragments from agarose gels
• The key to isolating undamaged DNA is the unique crystal violet reagent, which allows visualization of DNA (≥200 ng) in the gel under ambient light
• This eliminates the need for ethidium bromide and exposure to UV light, which can nick and damage DNA
• Nucleic acids up to 7 kb are efficiently purified from the gel using the convenient S.N.A.P.™ resin, provided ready to use in a spin column format
• The resin eliminates organic extractions, which generate hazardous waste
• In addition, there are no time-consuming ethanol precipitations
• Easy to Use The S.N.A.P.™ UV-Free Gel Purification Kit yields pure, intact DNA in an easy-to-perform, rapid procedure
• Simply: 1
• Prepare gel with crystal violet reagent and load samples in crystal violet loading buffer
• 2
• Visualize DNA band under ambient light and excise from agarose gel
• 3
• Add NaI solution (provided) and melt the agarose at 42-50°C
• 4
• Load sample onto the S.N.A.P.™ spin column
• 5
• Wash away contaminants
• 6
• Elute DNA in water
• The eluted DNA is highly pure, intact, and ready to use for cloning, sequencing, transformation, or transfection.