K210003
Invitrogen™ PureLink™ HiPure Plasmid Miniprep Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K210003-Each-of-1 | In Stock | ₹ 70,330.32 |
K210003 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 70,330.32
GST (18%): ₹ 12,659.458
Total Price: ₹ 82,989.778
Content And Storage
• 50 mL Resuspension Buffer (R3)• 550 μL RNase A• 50 mL Lysis Buffer (L7)• 40 mL Precipitation Buffer (N3)• 250 mL Equilibration Buffer (EQ1)• 500 mL Wash Buffer (W8)• 90 mL Elution Buffer (E4)• 15 mL TE Buffer• 100 HiPure ColumnsStore all components at room temperature.
Isolation Technology
Silica Spin Column
Scale
Mini
For Use With (Application)
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions
100 Preps
Product Line
PureLink™
Quantity
100 Preps
Target
BAC DNA, Plasmid DNA
Format
Kit
Sample Type
Bacterial Culture
Final Product Type
BAC DNA, Plasmid DNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Prep Scale
< 100 μg (Small-Scale) Plasmid DNA
Product Type
Plasmid MiniPrep Kit
Shipping Condition
Room Temperature
Test Time
2 hr.
Related Products
Description
- Description The PureLink HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without use of any organic solvents or cesium chloride, and contains Low endotoxin levels
- Plasmid DNA of High Purity PureLink HiPure Plasmid Miniprep Kit is designed to efficiently isolate plasmid DNA from E.coli in 1.5–2 hours Kit employs anion-exchange resin composed of small particles with a uniform pore size to purify plasmid DNA to a level equivalent to two passes through CsCl gradients Need for additional steps to remove contaminants such as phenol, chloroform, and CsCl are eliminated, minimizing exposure to and disposal of hazardous materials Efficient—Increased yields with low endotoxin Levels High Yield and Efficient Endotoxin Removal PureLink HiPure Plasmid Miniprep Kit allows purification of up to 30 μg of high quality plasmid DNA from 1–3 mL overnight E.coli cultures in approximately 1 hour, with an endotoxin level of 0.1–1 EU/μg, when cloning high copy number plasmids Typically, DNA isolated using the PureLink HiPure Plasmid Miniprep Kit has an A 260 /A 280 >1.80 when samples are diluted in Tris-HCl (pH 7.5) indicating that the DNA is reasonably free of proteins that could interfere with downstream applications Isolated DNA can be used for a Number of Downstream Applications: Purified DNA is suitable for downstream applications, including those requiring the highest purity DNA, such as mammalian transfections, automated and manual DNA sequencing, PCR amplification, in vitro transcription, bacterial cell transformation, cloning, and labeling Order Info Shipping Condition: Room Temperature
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Content And Storage: • 50 mL Resuspension Buffer (R3)• 550 μL RNase A• 50 mL Lysis Buffer (L7)• 40 mL Precipitation Buffer (N3)• 250 mL Equilibration Buffer (EQ1)• 500 mL Wash Buffer (W8)• 90 mL Elution Buffer (E4)• 15 mL TE Buffer• 100 HiPure ColumnsStore all components at room temperature.
Isolation Technology: Silica Spin Column
Scale: Mini
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions: 100 Preps
Product Line: PureLink™
Quantity: 100 Preps
Target: BAC DNA, Plasmid DNA
Format: Kit
Sample Type: Bacterial Culture
Final Product Type: BAC DNA, Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
Product Type: Plasmid MiniPrep Kit
Shipping Condition: Room Temperature
Test Time: 2 hr.
Content And Storage:
• 50 mL Resuspension Buffer (R3)• 550 μL RNase A• 50 mL Lysis Buffer (L7)• 40 mL Precipitation Buffer (N3)• 250 mL Equilibration Buffer (EQ1)• 500 mL Wash Buffer (W8)• 90 mL Elution Buffer (E4)• 15 mL TE Buffer• 100 HiPure ColumnsStore all components at room temperature.
Isolation Technology:
Silica Spin Column
Scale:
Mini
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions:
100 Preps
Product Line:
PureLink™
Quantity:
100 Preps
Target:
BAC DNA, Plasmid DNA
Format:
Kit
Sample Type:
Bacterial Culture
Final Product Type:
BAC DNA, Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Prep Scale:
< 100 μg (Small-Scale) Plasmid DNA
Product Type:
Plasmid MiniPrep Kit
Shipping Condition:
Room Temperature
Test Time:
2 hr.
Content And Storage: • 100 mL Resuspension Buffer (R3)• 550 μL RNase A• 100 mL Lysis Buffer (L7)• 100 mL Precipitation Buffer (N3)• 250 mL Equilibration Buffer (EQ1)• 500 mL Wash Buffer (W8)• 125 mL Elution Buffer (E4)• 15 mL TE Buffer• 25 HiPure Columns• 5 Column HoldersStore all components at room temperature.
Isolation Technology: Anion Exchange Resin
Scale: __
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions: 25 Preps
Product Line: PureLink™
Quantity: 25 Preps
Target: BAC DNA, Plasmid DNA
Format: Column
Sample Type: Bacterial Culture
Final Product Type: BAC DNA, Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
Product Type: Plasmid MidiPrep Kit
Shipping Condition: Room Temperature
Test Time: 2 hr.
Content And Storage:
• 100 mL Resuspension Buffer (R3)• 550 μL RNase A• 100 mL Lysis Buffer (L7)• 100 mL Precipitation Buffer (N3)• 250 mL Equilibration Buffer (EQ1)• 500 mL Wash Buffer (W8)• 125 mL Elution Buffer (E4)• 15 mL TE Buffer• 25 HiPure Columns• 5 Column HoldersStore all components at room temperature.
Isolation Technology:
Anion Exchange Resin
Scale:
__
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions:
25 Preps
Product Line:
PureLink™
Quantity:
25 Preps
Target:
BAC DNA, Plasmid DNA
Format:
Column
Sample Type:
Bacterial Culture
Final Product Type:
BAC DNA, Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Prep Scale:
100-200 μg (Medium-Scale) Plasmid DNA
Product Type:
Plasmid MidiPrep Kit
Shipping Condition:
Room Temperature
Test Time:
2 hr.
Content And Storage: • 200 mL Resuspension Buffer (R3)• 1.5 mL RNase A• 200 mL Lysis Buffer (L7)• 200 mL Precipitation Buffer (N3)• 2 × 250 mL Equilibration Buffer (EQ1)• 2 x 500 mL Wash Buffer (W8)• 250 mL Elution Buffer (E4)• 30 mL TE Buffer• 50 HiPure Columns• 10 Column HoldersStore all components at room temperature.
Isolation Technology: Anion Exchange Resin
Scale: __
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions: 50 Preps
Product Line: PureLink™
Quantity: 50 Preps
Target: BAC DNA, Plasmid DNA
Format: Column
Sample Type: Bacterial Culture
Final Product Type: BAC DNA, Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
Product Type: Plasmid MidiPrep Kit
Shipping Condition: Room Temperature
Test Time: 2 hr.
Content And Storage:
• 200 mL Resuspension Buffer (R3)• 1.5 mL RNase A• 200 mL Lysis Buffer (L7)• 200 mL Precipitation Buffer (N3)• 2 × 250 mL Equilibration Buffer (EQ1)• 2 x 500 mL Wash Buffer (W8)• 250 mL Elution Buffer (E4)• 30 mL TE Buffer• 50 HiPure Columns• 10 Column HoldersStore all components at room temperature.
Isolation Technology:
Anion Exchange Resin
Scale:
__
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions:
50 Preps
Product Line:
PureLink™
Quantity:
50 Preps
Target:
BAC DNA, Plasmid DNA
Format:
Column
Sample Type:
Bacterial Culture
Final Product Type:
BAC DNA, Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Prep Scale:
100-200 μg (Medium-Scale) Plasmid DNA
Product Type:
Plasmid MidiPrep Kit
Shipping Condition:
Room Temperature
Test Time:
2 hr.
Content And Storage: • 100 mL Resuspension Buffer (R3)• 550 μL RNase A• 100 mL Lysis Buffer (L7)• 100 mL Precipitation Buffer (N3)• 300 mL Equilibration Buffer (EQ1)• 2 × 300 mL Wash Buffer (W8)• 200 mL Elution Buffer (E4)• 30 mL TE Buffer• 10 HiPure Columns• 3 Column HoldersStore all components at room temperature.
Isolation Technology: Anion Exchange Resin
Scale: Maxi
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions: 10 Preps
Product Line: PureLink™
Quantity: 10 Preps
Target: BAC DNA, Plasmid DNA
Format: Column
Sample Type: Bacterial Culture
Final Product Type: BAC DNA, Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
Product Type: Plasmid MaxiPrep Kit
Shipping Condition: Room Temperature
Test Time: 2 hr.
Content And Storage:
• 100 mL Resuspension Buffer (R3)• 550 μL RNase A• 100 mL Lysis Buffer (L7)• 100 mL Precipitation Buffer (N3)• 300 mL Equilibration Buffer (EQ1)• 2 × 300 mL Wash Buffer (W8)• 200 mL Elution Buffer (E4)• 30 mL TE Buffer• 10 HiPure Columns• 3 Column HoldersStore all components at room temperature.
Isolation Technology:
Anion Exchange Resin
Scale:
Maxi
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions:
10 Preps
Product Line:
PureLink™
Quantity:
10 Preps
Target:
BAC DNA, Plasmid DNA
Format:
Column
Sample Type:
Bacterial Culture
Final Product Type:
BAC DNA, Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Prep Scale:
> 200 μg (Large-Scale) Plasmid DNA
Product Type:
Plasmid MaxiPrep Kit
Shipping Condition:
Room Temperature
Test Time:
2 hr.