K210005
Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K210005-Each-of-1 | In Stock | ₹ 53,047.20 |
K210005 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 53,047.20
GST (18%): ₹ 9,548.496
Total Price: ₹ 62,595.696
Content And Storage
• 200 mL Resuspension Buffer (R3)• 1.5 mL RNase A• 200 mL Lysis Buffer (L7)• 200 mL Precipitation Buffer (N3)• 2 × 250 mL Equilibration Buffer (EQ1)• 2 x 500 mL Wash Buffer (W8)• 250 mL Elution Buffer (E4)• 30 mL TE Buffer• 50 HiPure Columns• 10 Column HoldersStore all components at room temperature.
Isolation Technology
Anion Exchange Resin
Elution Volume
5 mL
For Use With (Application)
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions
50 Preps
Prep Scale
100-200 μg (Medium-Scale) Plasmid DNA
Product Type
Plasmid MidiPrep Kit
Purity
Transfection Grade
Target
BAC DNA, Plasmid DNA
Test Time
2 hr.
Format
Column
Sample Type
Bacterial Culture
Final Product Type
BAC DNA, Plasmid DNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Plasmid
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Product Line
PureLink™
Quantity
50 Preps
Shipping Condition
Room Temperature
System Type
PureLink™
Yield
350 μg
Related Products
Description
- Description The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E
- coli
- The Midiprep Kit protocol will typically yield 100–350 μg plasmid DNA from 15–25 mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients
- Labor and time saving- no need for extra steps to remove endotoxin and other contaminants High-purity, low-endotoxin product- pure enough for mammalian cell transfection Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs Anion-exchange chromatography for plasmid purification PureLink HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to level equivalent to two passes through CsCl gradients The resin combines excellent capacity with fast flow rate, high resolution, high yield, and efficient endotoxin removal Plasmid preparations can typically be completed in less than 2 hours Contaminant-free DNA Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A 260 /A 280 ratio of greater than 1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection Purification time is two hours Order Info Shipping Condition: Room Temperature
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Content And Storage: • 200 mL Resuspension Buffer (R3)• 1.5 mL RNase A• 200 mL Lysis Buffer (L7)• 200 mL Precipitation Buffer (N3)• 2 × 250 mL Equilibration Buffer (EQ1)• 2 x 500 mL Wash Buffer (W8)• 250 mL Elution Buffer (E4)• 30 mL TE Buffer• 50 HiPure Columns• 10 Column HoldersStore all components at room temperature.
Isolation Technology: Anion Exchange Resin
Elution Volume: 5 mL
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions: 50 Preps
Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
Product Type: Plasmid MidiPrep Kit
Purity: Transfection Grade
Target: BAC DNA, Plasmid DNA
Test Time: 2 hr.
Format: Column
Sample Type: Bacterial Culture
Final Product Type: BAC DNA, Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Product Line: PureLink™
Quantity: 50 Preps
Shipping Condition: Room Temperature
System Type: PureLink™
Yield: 350 μg
Content And Storage:
• 200 mL Resuspension Buffer (R3)• 1.5 mL RNase A• 200 mL Lysis Buffer (L7)• 200 mL Precipitation Buffer (N3)• 2 × 250 mL Equilibration Buffer (EQ1)• 2 x 500 mL Wash Buffer (W8)• 250 mL Elution Buffer (E4)• 30 mL TE Buffer• 50 HiPure Columns• 10 Column HoldersStore all components at room temperature.
Isolation Technology:
Anion Exchange Resin
Elution Volume:
5 mL
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions:
50 Preps
Prep Scale:
100-200 μg (Medium-Scale) Plasmid DNA
Product Type:
Plasmid MidiPrep Kit
Purity:
Transfection Grade
Target:
BAC DNA, Plasmid DNA
Test Time:
2 hr.
Format:
Column
Sample Type:
Bacterial Culture
Final Product Type:
BAC DNA, Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Plasmid:
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Product Line:
PureLink™
Quantity:
50 Preps
Shipping Condition:
Room Temperature
System Type:
PureLink™
Yield:
350 μg
Content And Storage: • 100 mL Resuspension Buffer (R3)• 550 μL RNase A• 100 mL Lysis Buffer (L7)• 100 mL Precipitation Buffer (N3)• 300 mL Equilibration Buffer (EQ1)• 2 × 300 mL Wash Buffer (W8)• 200 mL Elution Buffer (E4)• 30 mL TE Buffer• 10 HiPure Columns• 3 Column HoldersStore all components at room temperature.
Isolation Technology: Anion Exchange Resin
Elution Volume: 15 mL
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions: 10 Preps
Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
Product Type: Plasmid MaxiPrep Kit
Purity: Transfection Grade
Target: BAC DNA, Plasmid DNA
Test Time: 2 hr.
Format: Column
Sample Type: Bacterial Culture
Final Product Type: BAC DNA, Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Product Line: PureLink™
Quantity: 10 Preps
Shipping Condition: Room Temperature
System Type: PureLink™
Yield: 1000 μg
Content And Storage:
• 100 mL Resuspension Buffer (R3)• 550 μL RNase A• 100 mL Lysis Buffer (L7)• 100 mL Precipitation Buffer (N3)• 300 mL Equilibration Buffer (EQ1)• 2 × 300 mL Wash Buffer (W8)• 200 mL Elution Buffer (E4)• 30 mL TE Buffer• 10 HiPure Columns• 3 Column HoldersStore all components at room temperature.
Isolation Technology:
Anion Exchange Resin
Elution Volume:
15 mL
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions:
10 Preps
Prep Scale:
> 200 μg (Large-Scale) Plasmid DNA
Product Type:
Plasmid MaxiPrep Kit
Purity:
Transfection Grade
Target:
BAC DNA, Plasmid DNA
Test Time:
2 hr.
Format:
Column
Sample Type:
Bacterial Culture
Final Product Type:
BAC DNA, Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Plasmid:
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Product Line:
PureLink™
Quantity:
10 Preps
Shipping Condition:
Room Temperature
System Type:
PureLink™
Yield:
1000 μg
Content And Storage: • 250 mL Resuspension Buffer (R3)• 1.5 mL RNase A• 250 mL Lysis Buffer (L7)• 250 mL Precipitation Buffer (N3)• 2 x 400 mL Equilibration Buffer (EQ1)• 3 x 500 mL Wash Buffer (W8)• 400 mL Elution Buffer (E4)• 30 mL TE Buffer• 25 HiPure Columns• 5 Column HoldersStore all components at room temperature.
Isolation Technology: Anion Exchange Resin
Elution Volume: 15 mL
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions: 25 Preps
Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
Product Type: Plasmid MaxiPrep Kit
Purity: Transfection Grade
Target: BAC DNA, Plasmid DNA
Test Time: 2 hr.
Format: Column
Sample Type: Bacterial Culture
Final Product Type: BAC DNA, Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Product Line: PureLink™
Quantity: 25 Preps
Shipping Condition: Room Temperature
System Type: PureLink™
Yield: 1000 μg
Content And Storage:
• 250 mL Resuspension Buffer (R3)• 1.5 mL RNase A• 250 mL Lysis Buffer (L7)• 250 mL Precipitation Buffer (N3)• 2 x 400 mL Equilibration Buffer (EQ1)• 3 x 500 mL Wash Buffer (W8)• 400 mL Elution Buffer (E4)• 30 mL TE Buffer• 25 HiPure Columns• 5 Column HoldersStore all components at room temperature.
Isolation Technology:
Anion Exchange Resin
Elution Volume:
15 mL
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions:
25 Preps
Prep Scale:
> 200 μg (Large-Scale) Plasmid DNA
Product Type:
Plasmid MaxiPrep Kit
Purity:
Transfection Grade
Target:
BAC DNA, Plasmid DNA
Test Time:
2 hr.
Format:
Column
Sample Type:
Bacterial Culture
Final Product Type:
BAC DNA, Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Plasmid:
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Product Line:
PureLink™
Quantity:
25 Preps
Shipping Condition:
Room Temperature
System Type:
PureLink™
Yield:
1000 μg
Content And Storage: • Equilibration Buffer• Resuspension Buffer• Lysis Buffer• Precipitation Buffer• RNase A• Wash Buffer• Elution Buffer• TE Buffer• Expi HiPure Mega Filtration Column• Expi HiPure Mega DNA Binding ColumnStore all components at room temperature upon receipt.
Isolation Technology: Anion Exchange Resin
Elution Volume: __
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions: 4 Preps
Prep Scale: 4 mg plasmid DNA
Product Type: Plasmid MegaPrep Kit
Purity: __
Target: Plasmid DNA
Test Time: 2 hr.
Format: Kit
Sample Type: __
Final Product Type: __
High-throughput Compatibility: Manual Protocols
Plasmid: __
Product Line: PureLink™
Quantity: 4 Preps
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
System Type: __
Yield: __
Content And Storage:
• Equilibration Buffer• Resuspension Buffer• Lysis Buffer• Precipitation Buffer• RNase A• Wash Buffer• Elution Buffer• TE Buffer• Expi HiPure Mega Filtration Column• Expi HiPure Mega DNA Binding ColumnStore all components at room temperature upon receipt.
Isolation Technology:
Anion Exchange Resin
Elution Volume:
__
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions:
4 Preps
Prep Scale:
4 mg plasmid DNA
Product Type:
Plasmid MegaPrep Kit
Purity:
__
Target:
Plasmid DNA
Test Time:
2 hr.
Format:
Kit
Sample Type:
__
Final Product Type:
__
High-throughput Compatibility:
Manual Protocols
Plasmid:
__
Product Line:
PureLink™
Quantity:
4 Preps
Shipping Condition:
Approved for shipment at Room Temperature or on Wet Ice
System Type:
__
Yield:
__