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CAT reporter system

CheckMate™/Flexi™ vector mammalian two-hybrid system

Chroma-Glo assay system

Electroporator, Neon™ Transfection System

MultiSite Gateway™ Pro kits (e.g. cat. no. 12537-100)

Protease-Glo™ assay

Beta-Gal (lacZ)

Beta-Gal (lacZ), HIS3, URA3

BFP

BLA (Beta-Lactamase)

Firefly Luciferase (luc2), Renilla Luciferase (hRluc-neo)

GFP (Cycle 3)

GFP (EmGFP)

hRlucB

luc2A

luc2P

Luciferase

None

YFP

0.01 mg

1 Ea.

1 kit

1 Kit

1 L

1 mg

1 mL

1 Stab

1 tube

1 Vial

1 x 107cells

1 x 107cells/mL

1 μg

10 mL

10 x 100 mL

10 μg

100 mL

100 Reactions

100 μg

1000 Tests

1000 Units

2 x 0.55 mL

2 μg

20 reactions

20 Reactions

20 μg

200 Tests

20μg

25 μg

250 Units

40 Reactions

5 Reactions

5 transfections

5 x 40 μL

50 Columns

500 pmol

6 purifications

6 μg

60 reactions

9 x 2 μg

Sufficient for up to 10 transformations in each host

AOX1

AP1 RE

araBAD

ARE

CMV

CMV/TO

CRE

Dual Opposed T7/SP6

E1b (GAL4-E1b)

EF-1α

EF-1α, CMV

GAL1

GAL4UAS

GAP

HRE

HSV-TK

IE1

minP

MMTV

MT

NF-κB-RE

NFAT-RE

None (Promoterless)

None (Promoterless), EF-1α, CMV

OplE2

p10, Polyhedrin

PGK

Polyhedrin

Polyhedrin (2 copies), p10 (2 copies)

RSV, CMV

SP6, T7

SRE

SRF-RE

STAT5 RE

SV40

T7

T7, lacO

T7lac

Trc, lacO

UbC

XRE

-20°C

-30°C to -10°C

• BacMam pCMV-Dest Vector, 10 μg, store at 2°C to 8°C• MAX Efficiency™ DH10Bac™ Chemically Competent Cells, 0.5 mL, store at -68°C to -85°C• Cellfectin™ Reagent, 1 mL, store at 2°C to 8°C

• pAd/PL-DEST Gateway™ Vector (Store at -20°C)• 293A Cell Line (Store in liquid nitrogen)

• pAd⁄PL-DEST™ Vector 150 ng⁄μl in TEBuffer, pH 8.0. 40 μl (Store at -20°C)• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 μg⁄μl in TE Buffer, pH 8.0. 10 μl (Store at -20°C)

<-65°C

≤-70°C

1 vial (1 mg/mL) pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C

1 vial of 1 x 107cells. Store in liquid nitrogen.

1 x 107cells are supplied frozen in 1 ml 90% complete medium and 10% DMSO. Store in liquid nitrogen. Cells are guaranteed stable for 6 months when properly stored.

10 μg of BacMam pCMV-Dest Vector.Store at 2°C to 8°C.

10 μg pENTR™ Dual Selection vector, in 20 ul in TE buffer, pH 8.0.Store at -20°C

10 μg pENTR™ Dual Selection vector, lyophilized in TE, pH: 8.0. Resuspend in waterStore at -20°C

100 μg mifepristone is provided lyophilized. Store at -20°C. Mifepristone is guaranteed stable for 6 months when properly stored.

150 μg at 1 mg/mLStore at 2-8°C or long term storage at -20°C.

2 × Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)2 × Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA

2 × Box 1 (store at -20°C)• 20 μl pcDNA™3.1/V5-His-TOPO™ (10 ng/μl)• 100 μl 10X PCR Buffer• 10 μl dNTP Mix (50 mM)• 50 μl Salt Solution• 1 ml Sterile Water• 10 μl Control PCR Template (50 ng/μl)• 10 μl Control PCR Primers (100 ng/μl each)• 20 μl T7 Sequencing Primer (100 ng/μl)• 20 μl BGH Reverse Sequencing Primer (100 ng/μl)• 10 μl Expression Control Plasmid (500 ng/μl)2 × Box 2 (store at -80°C)• 21 × 50 μl TOP10E. colicells• 50 μl pUC19 Control DNA (10 pg/μl)• 6 ml S.O.C. Medium

20 μg each of pBudCE4.1 and pBudCE4.1/lacZ/CAT positive control vectors are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

20 μg each of pPICZ A, B, & C suspended in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. A GS115/pPICZ/lacZ Positive Control strain is also included.Store vectors at -5 to -30°C.

20 μg of each pRSET A, B, & C vector is provided lyophilized. TOP10F´, BL21(DE3)pLysS, and BL21 pRSET/lacZ control E. coli stabs are also included. Store E. coli stabs at 2–8°C. All components are guaranteed stable for 6 months when properly stored.

20 μg of each pVAX1 and expression control vector are supplied supercoiled and lyophilized. Store at -20°C. Vector is guaranteed stable for 6 months when properly stored.

20 μg of each vector and a PSA expression control are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

20 μg of pCEP4 vector in Tris-EDTA bufferStore at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

20 μg of this pcDNA™3.1(-)vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

20 μg of this pcDNA™3.1(+)vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

20 μg of this pcDNA™3.1/Hygro(+)vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

20 μg of this pcDNA™3.1/Zeo(-)vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

20 μg of this pcDNA™3.1/Zeo(+)vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

20 μg pDisplay vector is supercoiled and supplied as a 0.5 μg/mL solution in TE buffer, pH 8.0. Store at -20°C. Guaranteed stable for 6 months when properly stored.

250 or 1000 units of EKMax™ is provided with 10X buffer. Store at -20°C. Guaranteed stable for 6 months when stored properly.

3 DNA vectors mixed into 1 tube at 1 μg/μl in TE buffer

3 x 106cells are supplied frozen in 1 ml of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen. Cells are guaranteed stable for 6 months when properly stored.

6 μg pDONR221™ vector, lyophilized in TE buffer, pH 8.0. Upon receipt, store at -20°C.

6 μg pDONR™/Zeo vector, lyophilized in TE buffer, pH 8.0. pDONR™/Zeo is also supplied with 1.25 ml Zeocin™. Zeocin™ is provided as a 100 mg/ml solution in deionized, sterile water. Upon receipt, store the pDONR™/Zeo vector at -20°C and the Zeocin™ at -20°C, protected from light.

All destination vectors are provided lyophilized and supercoiled.

All vectors are supercoiled and are provided lyophilized (store at -20°C). AlacZpositive expression control strain is provided with the pGAPZ vector.Store strains at 4°C.All reagents are guaranteed stable for 6 months when properly stored.

All vectors are supercoiled and are provided lyophilized. A TOP10E. colistab and positive expression control are provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored.

Below -10°C

Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA

Box 1 (store at -20°C)• 20 μl pcDNA™3.1/V5-His-TOPO™ (10 ng/μl)• 100 μl 10X PCR Buffer• 10 μl dNTP Mix (50 mM)• 50 μl Salt Solution• 1 ml Sterile Water• 10 μl Control PCR Template (50 ng/μl)• 10 μl Control PCR Primers (100 ng/μl each)• 20 μl T7 Sequencing Primer (100 ng/μl)• 20 μl BGH Reverse Sequencing Primer (100 ng/μl)• 10 μl Expression Control Plasmid (500 ng/μl)Box 2 (store at -80°C)• 21 × 50 μl TOP10E. colicells• 50 μl pUC19 Control DNA (10 pg/μl)• 6 ml S.O.C. Medium

Box 1 (store at -20°C)• 20 μl pcDNA™3.3-TOPO™ vector, TOPO™ adapted (5–10 ng/μl)• 100 μl 10X PCR Buffer• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 10 μl Control PCR Template (50 ng/μl)• 10 μl Control PCR Primers (100 ng/μl each)• 20 μl CMV Forward Sequencing Primer (100 ng/μl)• 20 μl TK polyA Reverse Sequencing Primer (100 ng/μl)• 10 μl pcDNA™3.3-TOPO™/lacZ Expression Control Plasmid (500 ng/μl)Box 2 (store at -80°C)• 11 × 50 μl TOP10E. coli• 50 μl pUC19 Control DNA (10 pg/μl)• 6 ml S.O.C. Medium

Component A (1X liquid) and Component B (liquid)Store in the dark at 2−8°C.Shelf life: 12 months from date of manufacture

Contains 20 μg of each: pcDNA™FRT⁄TO and pcDNA™FRT⁄TO⁄CAT vectors. Store both vectors at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

Contains 5 vials of linear pUC19L vector at a concentration of 50 ng/mL. Each vial contains enough plasmid for 4 cloning reactions using the GeneArt™ Seamless Cloning and Assembly Kit (Cat. No. A13288, must be purchased separately).Store the product at -80°C.Vector is guaranteed stable for 6 months when properly stored.

Contains both pcDNA5⁄FRT and pcDNA5⁄FRT⁄CAT vectors. Store at -20°C

ContentsThe ViraPower™ Bsd Lentiviral Support Kit includes the following components:• 195 μg of ViraPower™ Packaging Mix (Contains 195 μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1 μg⁄μL in TE Buffer, pH 8.0)• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)• 50 mg of Blasticidin powder (Cat # R21001)Storage• ViraPower™ Packaging Mix: -20°C• Lipofectamine™ 2000: +4°C (do not freeze)• Blasticidin: -20°C

ContentsThe ViraPower™ Zeo Lentiviral Support Kit includes the following components:• 195 μg of ViraPower™ Packaging Mix (Contains 195 μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1 μg⁄μL in TE Buffer, pH 8.0)• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)• 125 mg of Zeocin™ (1.25 ml of a 100mg⁄ml solution)Storage• ViraPower™ Packaging Mix: -20°C• Lipofectamine™ 2000: 4°C (do not freeze)• Zeocin™: -20°C, protected from light

CT-GFP Fusion Expression kit contain two boxes: 1. TOPO™ cloning box with pcDNA™3.1⁄CT-GFP TOPO™ cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. 2. One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store at -80°C. Kits are guaranteed stable for 6 months when properly stored

Each Bac-to-Bac Baculovirus Expression System includes:• pFastBac1 vector (10 μg), store at -20°C• pFastBac 1-Gus control vector (4 ng), store at -20°C• MAX Efficiency DH10Bac Chemically CompetentE. coli(1 kit with 5 x 0.1 mL), store at -80°C• ExpiFectamine Sf Transfection Reagent (1 mL), store at 4°C, do not freeze

Each Champion™ pET Directional TOPO™ Expression Kit is provided as a complete expression system. The Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C. The One Shot™ TOP10 box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot™ BL21 Star™(DE3) box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. Kits with Lumio™ Technology include 20 μl of the Lumio™ Detection Reagent. Store at -20°C. Guaranteed stable for 6 months when properly stored.

Each DES™-Inducible Kit contains 20 μg each of a selection vector (pCoHygro or pCoBlast), pMT/V5-His A, B, C, and pMT/V5-His/lacZ,Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), frozen S2 cells, GIBCO™ Schneider'sDrosophilaMedium, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.

Each kit contains the following components:• pFRT/lacZeo: 20 μg, lyophilized in TE• pcDNA6/TR: 20 μg, lyophilized in TE• pOG44: 20 μg, lyophilized in TE• pcDNA5/FRT/TO: 20 μg, lyophilized in TE• pcDNA5/FRT/TO/CAT: 20 μg, lyophilized in TE• CMV Forward Primer (21-mer): 2 μg, lyophilized in TE• BGH Reverse Primer (18-mer): 2 μg, lyophilized in TE• Tetracycline: 5 gThe kit ships at room temperature. Store vectors and primers at -20°C. Store tetracycline at 4°C, protected from light.

Each kit includes:• Expression vector in A, B, & C versions, 20 μg/vector (store at -20°C)•lacZ (intracellular) or HSA (secreted) control vector, 20 μg (-20°C)• X-33Pichiastab (4°C)The Starter Kits also include 50 mg of Blasticidin, store at -20°C.All reagents are guaranteed stable for 6 months when properly stored.

Each pBAD TOPO™ TA Expression Kit contains two boxes and the LMG194E. colistab. The pBAD TOPO™ TA box contains all of the reagents necessary for PCR (exceptTaqpolymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation, including single-use 50 μl aliquots of One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ cells at -80°C. Store the LMG194E. colistab at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.

Each pBAD TOPO™ TA Expression Kit contains two boxes and the LMG194E. colistab. The pBAD TOPO™ TA box contains all of the reagents necessary for PCR (exceptTaqpolymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation, including single-use 50 μl aliquots of One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ cells at -80°C. Store the LMG194E. colistab at at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.

Each pBAD/TOPO™ ThioFusion™ Expression Kit contains two boxes and the LMG194E. colistab. The pBAD/TOPO™ ThioFusion™ Expression box contains all of the reagents necessary for PCR (exceptTaqpolymerase) including 200 ng of topoisomerase I-activated pBAD/Thio-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation including 21 single-use 50 μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store the One Shot™ box at -80°C. Store the LMG194E. colistab at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.

Each pcDNA™6.2-TOPO™ Expression Vector Kit contains two boxes. The TOPO™ box contains linearized and topoisomerase I-activated pcDNA™6.2-TOPO™ vector, control template, primers for sequencing, PCR reaction components, and an expression control plasmid. The TOPO™ box should be stored at -20°C. The One Shot™ box contains transformation reagents including single-use 50 μl aliquots of One Shot™ TOP10 chemically competentE. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store competentE. coliat -80°C. All reagents are guaranteed stable for 6 months when properly stored.

Each pEF6/V5-His TOPO™ TA Expression Kit contains two boxes. The TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pEF6/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control templates and primers, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Competent Cell box contains all of the reagents necessary for transformation, including twenty-one 50-μl aliquots of TOP10 Chemically CompetentE. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store at -80°C. Guaranteed stable for 6 months when properly stored.

Each pIB/V5-His TOPO™ TA Expression Kit contains two boxes. The pIB/V5-His TOPO™ box contains 200 ng of linearized and topoisomerase I-activated pIB/V5-His-TOPO™ vector, dNTPs, 10X PCR buffer, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, salt solution, and pIB/V5-His/CAT. Store at -20°C. The One Shot™ box contains high-efficiency, single-use, 50 μl aliquots of chemically competent TOP10E. colicells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. All reagents are guaranteed stable for 6 months when properly stored.

Each pYES2.1 TOPO™ TA Expression Kit contains two boxes. The pYES2.1 TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pYES2.1/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers,GAL1Forward and V5 C-term Reverse Primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Box contains all of the reagents necessary for transformation, including twenty-one 50 μl aliquots of chemically competent TOP10F´E. coli, S.O.C. medium, and supercoiled plasmid control pUC19. Store at -80°C. All components are guaranteed stable at the indicated temperature for 6 months when properly stored.

Each vector is supplied as supercoiled and lyophilized plasmid DNA. Store at -20°C after resuspended. Guaranteed stable for 6 months when properly stored.

EKMax™ Enterokinase is provided with 10X buffer. Store at -20°C. Guaranteed stable for 6 months when stored properly.

GFP Fusion TOPO™ Expression Kits contain two boxes. The GFP Fusion TOPO™ TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-μl aliquots of One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot™ box at -80°C. Kits are guaranteed stable for 6 months when properly stored.

Immediately upon receipt, store vector at -20°C

pAd⁄CMV⁄V5-DEST Gateway™ Vector: store at -20°C;.293A Cells: store in liquid nitrogen.Guaranteed stable for 6 months when properly stored.

pDEST™ 17 Vector is supplied in TE buffer (pH 8.0) at 150ng/μl. Store at -20°C. Guaranteed for 6 months if stored properly.

pFastBac™ Dual (10 μg) and pFastBac™ Dual Control vector (4 ng) are supplied lyophilized.Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.

PichiaPink™ Secreted Protein Vector kit contains 20 μg pPinkα-HC (the high-copy number plasmid with the S. cerevisiae Alpha mating factor presequence) with sequencing primers 5' α-factor and 3' CYC1. Store at -20°C.

pJTI™ R4 CMV-TO MCS pA vector (100 μg), store at -20 °C

pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C

pLenti6.2-GW⁄EmGFP Vector: 20 μg of control vector is supplied in solution as 40 μl of 0.5 μg⁄μl in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. Store at –20°C.

pLenti6.2⁄V5-DEST™ Gateway™ Vector Box contains pLenti6.2⁄V5-DEST, 40 μl of 150 ng⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0 and pLenti6.2⁄V5-GW⁄lacZ control, 20 μl of 0.5 μg⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0. Store at –20°C.One Shot™ Stbl3™ chemically competentE. colibox contains transformation reagents including 20 single-use 50 μl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.

pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit• 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0)• 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)• One Shot™ Stbl3™ Chemically CompetentE. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL).ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)• 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0)• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)• 50 mg of Blasticidin powder (Cat # R21001)• 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106frozen cells in 1 mL of Freezing Medium.Storage• Vectors: -20°C• ViraPower™ Packaging Mix: -20°C• Blasticidin: -20°C• Lipofectamine™ 2000: 4°C (do not freeze)• One Shot™ Stbl3™ Chemically CompetentE. coli:-80°C• 293 FT Cells: Liquid Nitrogen

pLenti6.3/V5-GW/EmGFP vector: 20 μg (40 μL of 0.5 μg/μL vector in TE buffer, pH 8.0)Store at-20°C.

pLenti6.4/R4R2/V5-DEST™ Kit• pLenti6.4/R4R2/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti6.4/CMV/V5-MSGW/lacZ plasmid (20 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/CMVp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/EF1αp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μgStore at -20°CpENTR 5' TOPO™ TA Cloning Kit(Cat. No. K95120)• pENTR 5' TOPO™ Reagents, store at -20°C.• One Shot™ TOP10 Chemically Competent E.coli (Cat. No. C404003), store at -80°C.One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C

pLenti6⁄V5-DEST™ Gateway™ Vector KitpLenti6⁄V5™ vectors: -20°COne Shot™ Stbl3™ Chemically CompetentE. coli: -80°CViraPower™ Bsd Lentiviral Support KitViraPower™ Packaging Mix –20°CLipofectamine™ 2000 +4°C (DO NOT freeze)Blasticidin: -20°C293FT Cell Line: liquid nitrogen

pLenti6⁄V5™ Directional TOPO™ Cloning Kit:• pLenti6⁄V5™ Directional TOPO™ Reagents: -20°C• One Shot™ Stbl3™ Chemically CompetentE. coli:-80°CViraPower™ Bsd Lentiviral Support Kit:• ViraPower™ Packaging Mix: -20°C• Lipofectamine™ 2000: 4°C (DO NOT freeze)• Blasticidin: -20°C• 293FT Cell Line: liquid nitrogen

pLenti7.3/V5-DEST™ Gateway™ Vector Kit• pLenti7.3/V5-DEST™ Gateway™ Vector (150 ng/μL), 40 μL• pLenti7.3/V5-GW/lacZ Control Vector (0.5 μg/μL) , 20 μlLStore at -20°C.ViraPower™ Lentiviral Support KitContains enough reagents for 20 transfections in 10 cm tissue culture dishes:• Lyophilized Packaging Mix, 195 μg, store at -20°C• Lipofectamine™ 2000, 0.75 mL, store at 4°COne Shot™ Stbl3™ chemically competentE. colibox• One Shot™ Competent Cells, 20 single-use aliquots of 50 μL• S.O.C. medium• pUC19 supercoiled plasmid controlStore at -80°C.293FT cell line• 3 x 106frozen cells in 1 mL of Freezing Medium, store in liquid nitrogen.

pLenti7.3/V5-DEST™ Gateway™ Vector Kit• pLenti7.3/V5-DEST™ Gateway™ Vector (150 ng/μL), 40 μL• pLenti7.3/V5-GW/lacZ Control Vector (0.5 μg/μL), 20 μLStore at -20°C.One Shot™ Stbl3™ Chemically CompetentE. coliboxTransformation reagents including:• One Shot™ Competent Cells, 20 x 50 μL single-use aliquots• S.O.C. medium• pUC19 supercoiled plasmid controlStore at -80°C.

Ponasterone A is provided as a lyophilized powder. Store at -20°C. Ponasterone A is guaranteed stable for 6 months when properly stored.

Six 2-ml resin columns and buffers for native and denaturing purification. Twelve milliliters of ProBond™ pre-charged resin. Store at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

Store at _70°C. Avoid freeze/thaw cycles.

Store at - 70°C. Avoid freeze/thaw cycles.

Store at -20°C

Store at -20°C.

Store at -20°C. Avoid freeze-thaw cycles.

Store at -20°C. Avoid freeze/thaw cycles.

Store at -20°C. Ok to freeze.

Store at -70°C.

Store at -70°C. Avoid freeze/thaw cycle.

Store at -70°C. Avoid freeze/thaw cycles

Store at -70°C. Avoid freeze/thaw cycles.

Store at +4°C. Store at -20°C long term.

Store at ≤70°C

Store at ≤70°C.

Store at 2° to 8°.

Store at 2° to 8°C.

Store at or below -70°C.

Store at room temperature.

Store Human IgG1 Expression Control at -20°C

Store in freezer (-5 to -30°C).

Store in ultra-cold freezer (-68 to -85°C).

Store mat -70°C. Avoid freeze/thaw cycles.

Store pcDNA5/TO at -20°C

Store vector at -20°C immediately upon receipt.

The Bac-N-Blue™ Transfection Kit contains five 0.5 μg vials of Bac-NBlue™ linear virus DNA and 125 μl of Cellfectin™ Reagent.Store at +4°C. All components are guaranteed stable for 6 months when properly stored.

The BaculoDirect expression kits include five 0.3 µg vials of BaculoDirect Linear DNA, 125 µL of Cellfectin transfection reagent, frozen Sf9 cells, Gateway LR Clonase enzyme mix, GIBCO Unsupplemented Grace's Insect Medium, and ganciclovir.Store the BaculoDirect Linear DNA, Cellfectin reagent, and Grace's Medium at +4°C. Store LR Clonase mix at -80°C. Store Sf9 cells in liquid nitrogen. All components are guaranteed stable for 6 months if stored properly.

The BaculoDirect™ transfection kits include five 0.3 μg vials of BaculoDirect Linear DNA, 125 μL Cellfectin transfection reagent, and ganciclovir.Store the BaculoDirect Linear DNA and Cellfectin at 4°C. All components are guaranteed stable for 6 months if stored properly.

The Baculovirus Expression System with Gateway™ Technology includes LR Clonase™ enzyme mix and reaction buffer, destination vectors pDEST™8, pDEST™10, and pDEST™20, proteinase K solution (2 μg/μl), pENTR™-gus positive control, and Library Efficiency™ DH5Competent Cells. The kit does not include MAX Efficiency™ DH10Bac™ Competent Cells. Store LR Clonase™ enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored.

The Champion™ pET SUMO Expression System is provided as a complete system. The Champion™ pET SUMO TA Cloning™ box contains linearized Champion™ pET SUMO vector, sterile water, dNTPs, 10X PCR Buffer, control template and primers, T4 DNA ligase, 10X ligation buffer, primers for sequencing or PCR screening, and an expression control. Store at -20°C. The SUMO Protease box contains SUMO Protease and buffers. Store at -80°C. The One Shot™ TOP10 box contains twenty-one 50 μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot¤ BL21(DE3) box contains twenty-one 50 μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C.

The Champion™ pET-DEST42 Gateway™ destination vector includes 6 μg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.

The DES™ Inducible/Secreted Kits contains 20 μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), GIBCO™ Schneider'sDrosophilaMedium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.

The DES™-Blasticidin Support Kit contains 20 μg of pCoBlast, the Calcium Phosphate Transfection Kit, frozen S2 cells, GIBCO™ Schneider'sDrosophilaMedium, and 50 mg of Blasticidin. Store S2 cells in liquid nitrogen. Store medium at +4°C. Store all other reagents at -20°C. All components are guaranteed for 6 months when properly stored.

The Expressway™ Lumio™ Expression and Detection Systemincludes theE. coli slyD-Extract, 2.5X IVPSE. coliReaction Buffer, T7 Enzyme Mix, the Feed Buffer, 19 amino acid mix, Methionine, a control plasmid, DNAse/RNAse-free Water, and a Lumio™ Green Detection Kit for 20 real-time reactions or 100 in-gel applications. In addition to these components, the N-termExpressway™ Lumio™kit also includes 6 μg of the pEXP3-DEST vector and the C-termExpressway™ Lumio™kit 6 μg of the pEXP4-DEST vector. Store the vectors and the detection reagent at -20°C. Store all other components at -80°C. Guaranteed stable for 6 months when properly stored.

The Flp-In™ Complete System includes the Core Kit (K601002), 1 g Zeocin™ antibiotic, and 1 g hygromycin B. All vectors are supplied supercoiled and lyophilized. Store hygromycin at +4°C. Store all other components at -20°C. Zeocin™ antibiotic should be protected from exposure to light. All reagents are guaranteed stable for 6 months when properly stored.

The Flp-In™ Core Kit includes 20 μg each of pFRT⁄lacZeo, pOG44, pcDNA™5⁄FRT, and pcDNA™5⁄FRT⁄CAT (expression control), and 2 μg each of forward and reverse sequencing primers. Store at -20C.

The Gateway™ Vector Conversion System contains Gateway™ Reading Frame Cassettes A, B, and C.1, pENTR™-gus positive control, and One Shot™ccdBSurvival Cells. Store DNA at -20°C and competent cells at -80°C.

The GeneSwitch™ System Core Kit includes 20 μg of each of the following vectors: pGene/V5-His (A, B, and C), the expression control vector pGene/V5-His/lacZ, and the pSwitch vector. The kit also includes forward and reverse sequencing primers and 100 μg of the inducing agent mifepristone. The GeneSwitch™ Complete Kit includes the Core Kit with 1 g hygromycin and 1 g Zeocin™ at considerable savings. Hygromycin should be stored at +4°C and Zeocin™ at -20°C. Zeocin™ should be protected from exposure to light. All reagents are stable for 6 months when properly stored.

The InsectSelect pIB/V5-His Vector Kit includes 20 μg each of supercoiled lyophilized pIB/V5-His and pIB/V5-CAT vectors, and 2 μg lyophilized OplE2 Forward and Reverse sequencing primers.Store all reagent at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

The InsectSelect™ Glow Kit includes 20 μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 μg each of lyophilizedOpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin™ Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

The InsectSelect™ Kit includes 20 μg each of supercoiled, lyophilized pIZ/V5-His and pIZ/V5-His/CAT, 2 μg of lyophilizedOpIE2 Reverse Sequencing primer, 1 g Zeocin™, Cellfectin™ Reagent, and 1 L of medium for the insect cell line chosen. Sf9 cells are supplied with GIBCO™ Grace's Insect Media, High Five™ Cells are supplied with GIBCO™ Express Five™ Serum-Free Medium. The pIZ/V5-His Vector Kit contains only the expression vectors and primer as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

The MultiSite Gateway™ Pro Kits include vectors and primers as indicated in Table 1, One Shot™ Mach1™ T1RChemically Competent Cells, and LR Clonase™ II Plus and BP Clonase™ II enzymes. Store cells at -80°C. Store Clonase™ enzymes at -20°C for up to 6 months, or at -80°C for long-term storage. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

The pBAD/gIII Kit includes 20 μg each of pBAD/gIII A, B, & C, 20 μg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10E. colistabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.

The pBAD/His Kit includes 20 μg of each pBAD/His A, B, & C vector, 20 μg of pBAD/His/lacZ, 1 ml of 20% L-arabinose, LMG194 and TOP10E. colistabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.

The pBAD/Myc-His Kit includes 20 μg of pBAD/Myc-His A, B, & C vector, 20 μg of pBAD/Myc-His/lacZ, 20% L-arabinose, LMG194 and TOP10E. colistabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.

The pcDNA™4/HisMax A, B, & C vectors, 20 μg each, are supercoiled and provided lyophilized. 20 μg of pcDNA™4/HisMax/lacZ control plasmid is also provided. Store vectors at -20°C. The pcDNA™4/HisMax TOPO™TA Expression Kit contains two boxes. The TOPO™ TA box contains 200 ng of linearized and topoisomerase I-activated pcDNA4/HisMax-TOPO™ vector, dNTPs, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and alacZ expression control plasmid.Store this box at -20°C. The One Shot™ box contains transformation reagents including twenty-one 50-μl aliquots of TOP10E. coli, S.O.C. medium, and a pUC19 supercoiled control plasmid. The One Shot™ box should be stored at -80°C. Guaranteed stable for 6 months when properly stored.

The pENTR™ 5´-TOPO™ TA Cloning™ Kit with One Shot™ TOP10 Chemically CompetentE. colicontains two boxes.The TOPO™ Cloning box contains enough linearized and topoisomerase I-activated pENTR™ 5´-TOPO™ vector for 20 cloning reactions, dNTPs, salt solution, control template and primers and primers for sequencing. Store at -20°C.The One Shot™ TOP10 box contains transformation reagents including 20 single-use 50 μl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.

The pMIB/V5-His Vector Kit contains 20 μg each of supercoiled, lyophilized pMIB/V5-His A, B, and C vectors, pMIB/V5-His/CAT control vector, and 2 μg each of theOpIE2 Forward and Reverse Sequencing primers. Store at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

The pSCREEN-iT™/lacZ-DEST Gateway™ Vector Kit contains 6 μg of vector and 10 μg of pSCREEN-iT™/lacZ-GW/CDK2 control vector, the positive control Stealth™RNAilacZ, a negative control Scrambled Stealth RNAi™ siRNA, and 1X annealing/dilution buffer. Store at -20°C. The BLOCK-iT™ RNAi Target Screening Kit contains the pSCREEN-iT™/lacZ-DEST Gateway™ Vector Kit, 250 μl of Lipofectamine™ 2000 Reagent, and the FluoReporter™lacZ/Galactosidase Quantification Kit. Store the Lipofectamine™ 2000 Reagent at +4°C. Store the FluoReporter™ Kit at -20°C protected from light. The BLOCK-iT™ RNAi Target Screening System contains the BLOCK-iT™ RNAi Target Screening Kit, LR Clonase™ enzyme mix, and pCR ¤ 8/GW/TOPO ¤ TA Cloning Kit. Store the LR Clonase™ enzyme mix at -80°C. The pCR™ 8/GW/TOPO™ TA Cloning Kit includes the ready-to-use pCR™ 8/GW/TOPO™ vector, dNTPs, salt solution, water, forward and reverse sequencing primers, control template and PCR primers, One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and a pUC19 control plasmid. Store the Competent Cells at -80°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.

The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits each contain two boxes. The TOPO™ TA box contains 200 ng of linearized, topoisomerase I-activated pTrcHis-TOPO™ or pTrcHis2-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The One Shot™ TOP10 box contains twenty-one, 50-μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the TOPO TA Cloning™ box at -20°C. Store the One Shot™ box at -80°C. All reagents are guaranteed stable for 6 months when properly stored.

The pYES2 vector kit includes 20 μg of expression vector, a TOP10F´E. colistab, and an INVSc1 yeast stab. Store the vector at -20°C. Store the TOP10F´ and INVSc1 stabs at +4°C. All reagents are guaranteed stable for 6 months when stored properly.

The pYES2-DEST52 Gateway™ destination vector is provided supercoiled and lyophilized. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.

The Spheroplast Kit contains the following reagents: SOS medium, SE, SCE, 1M sorbitol, CaS, 40% PEG, CaT, sterile water, zymolyase, and 1M DTT. Sufficient reagents are provided in the Spheroplast Kit for 10 spheroplast preparations, each of which can be used for 5 transformations. All components are guaranteed stable for 6 months when properly stored.

The T-REx™ Core Kit includes 20 μg each of pcDNA™4/TO/myc-His A, B, and C; a positive control vector; pcDNA™6/TR; and forward and reverse sequencing primers.All vectors are supercoiled and provided lyophilized.Store all components at -20°C. All components are guaranteed stable for 6 months when properly stored.

The T-REx™ Core Kit includes 20 μg of pcDNA™4/TO or 20 μg each of pcDNA™4/TO/myc-His A, B, and C; a positive control vector, pcDNA™6/TR, and forward and reverse sequencing primers.All vectors are supercoiled and provided lyophilized.Store all components at -20°C. All components are guaranteed stable for 6 months when properly stored.

The T-REx™ Core Kit includes 20 μg of pcDNA™4/TO, a positive control vector, pcDNA™6/TR, and forward and reverse sequencing primers.The T-REx™ Complete Kit includes the Core Kit plus 1 g Zeocin™ (100 mg/ml), 50 mg of Blasticidin, and 5 g of tetracycline.All vectors are supercoiled and provided lyophilized.Store all components at -20°C. Zeocin™ and tetracycline should be protected from exposure to light. All components are guaranteed stable for 6 months when properly stored.

ThePichiaEasyComp™ Transformation Kit contains sufficient reagents (Solutions I, II, and III) for 6 preparations of competent cells. Each competent cell preparation yields enough cells for 20 transformations. Store Solutions I and III at +4°C. Store Solution II at room temperature. All components are guaranteed stable for 6 months when properly stored.

TheS.c.EasyComp™ Transformation Kit provides enough reagents to prepare 6 batches of competent cells. Each batch is sufficient for 20 transformations. The kit should be stored at +4°C. All components are guaranteed stable for 6 months when properly stored.

Three tubes, each containing 195 μg lyophilized mix. The packaging mix is enough for 60 transfection reactions in 10 cm tissue culture dishes. Store at -20°C.

Vector Kit (Box 1)pLenti6.3⁄V5-DEST™ (150 ng⁄μL): 6 μg, store at -20°CpLenti6.3⁄V5-GW⁄lacZ Control vector (500 ng⁄μL): 10 μg, store at -20°COne Shot™ Stbl3™ Chemically CompetentE. coli(Box 2)Stbl3™ Cells: 21 x 50 μL, store at -80°CpUC19 control DNA (10 pg⁄μL): 50 μL, store at -80°CS.O.C. Medium: 6 mL, store at -80°C

Vector Kit (Box 1)pLenti6⁄UbC⁄V5-DEST™:6 μg, store at -20°CpLenti6⁄UbC⁄V5-GW⁄lacZ Control vector: 10 ug, store at -20°COne Shot™ Stbl3™ Chemically CompetentE. coli(Box 2)Stbl3™ Cells: 21 x 50 μL, store at -80°CpUC19 control DNA (10 pg⁄μL): 50 μL, store at -80°CS.O.C. Medium: 6 mL, store at -80°C

Vector kit (Boxes 1 and 2)pLenti6⁄UbC⁄V5-DEST™ (Box 1): 6 μg, -20°CpLenti6⁄UbC⁄V5-GW⁄lacZ Control vector (Box 1): 10 μg, -20°COne Shot™ Stbl3™ Chemically CompetentE. coli(Box 2): 21 x 50 μL, -80°CpUC19 control DNA (10 pg⁄μL): 50 μL, -80°CS.O.C. Medium: 6 mL -80°CSupport kit (Box 3)ViraPower™ Packaging Mix (1 μg⁄μL): 195 μg, -20°CLipofectamine™ 2000: 0.75 mL, +4°CBlasticidin: 50 mg, -20°C293 FT cells (3 x 106⁄mL) (Box 4): 1 mL, liquid nitrogen

Vectors (and appropriate expression control) are supplied supercoiled and lyophilized. Stored at -20°C. All vectors are guaranteed stable for 6 months when properly stored.

Vectors are supplied supercoiled and lyophilized. pYES2/NT is provided in three reading frames. Both pYES/NT and pYES/CT include alacZ expression control vector and an INVSc1 yeast stab. Store vectors at -20°C. Store the stab at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit• pLenti6.3/TO/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C and protect from light.Store at -20°C unless otherwise notedOne Shot™ Stbl3™ Chemically CompetentE. coli(Cat. No. C737303)• Stbl3™ Cells in 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C.

ViraPower™ Lentiviral Gateway™ Vectors store at–20°C includes:pLenti4⁄V5-DEST™: 6 μg (40 μl of 150 ng⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0)pLenti4⁄V5-GW⁄lacZ control: 10 μg (20 μl of 0.5 μg⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0)One Shot™ Stbl3™ Chemically CompetentE. colistore at –80°C

ViraPower™ Lentiviral Gateway™ Vectors, store at –20°C includes:pLenti6⁄V5-DEST™: 6 μg (40 μl of 150 ng⁄μl vector in 10 mMTris-HCl, 1mM EDTA, pH 8.0)pLenti6⁄V5-GW⁄lacZ control: 10 μg(20 μl of 0.5 μg⁄μl vector in 10 mMTris-HCl, 1mM EDTA, pH 8.0)One Shot™ Stbl3™ Chemically CompetentE. colistore at –80°C