10-359-016
Invitrogen™ Bac-to-Bac™ Baculovirus Expression System
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 10-359-016-Each-of-1 | In Stock | ₹ 1,52,546.00 |
10-359-016 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,52,546.00
GST (18%): ₹ 27,458.28
Total Price: ₹ 1,80,004.28
Promoter
Polyhedrin
Content And Storage
Each Bac-to-Bac Baculovirus Expression System includes:• pFastBac1 vector (10 μg), store at -20°C• pFastBac 1-Gus control vector (4 ng), store at -20°C• MAX Efficiency DH10Bac Chemically CompetentE. coli(1 kit with 5 x 0.1 mL), store at -80°C• ExpiFectamine Sf Transfection Reagent (1 mL), store at 4°C, do not freeze
Cloning Method
Restriction Enzyme/MCS
Vector
pFastBac
Product Type
Baculovirus Expression System
Protein Tag
Untagged
Quantity
1 kit
Product Line
Bac-to-Bac™
Related Products
Description
- Relies on generation of recombinant baculovirus by site-specific transposition in E
- coli rather than homologous recombination in insect cells Time-saving expression bacmid: expression cassette of pFastBacvector recombines with parent bacmid in DH10Bac E
- coli Competent Cells to form expression bacmid Bacmid is then transfected into insect cells for production of recombinant baculovirus particles Easy colony screening Parent bacmid in DH10Bac E
- coli contains segment of lac Zα gene lac Zα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinants Flexibility Comes with pFastBac1 vector that offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloning Two other vectors are offered separately pFastBacHT vector for production of histidine-tagged recombinant protein and pFastBacDual vector for expression of two proteins simultaneously Rapid purification of recombinant proteins Optional pFastBacHT vector produces histidine-tagged recombinant protein for rapid protein purification with nickel-chelating resin Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
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Promoter: Polyhedrin
Content And Storage: Each Bac-to-Bac Baculovirus Expression System includes:• pFastBac1 vector (10 μg), store at -20°C• pFastBac 1-Gus control vector (4 ng), store at -20°C• MAX Efficiency DH10Bac Chemically CompetentE. coli(1 kit with 5 x 0.1 mL), store at -80°C• ExpiFectamine Sf Transfection Reagent (1 mL), store at 4°C, do not freeze
Cloning Method: Restriction Enzyme/MCS
Vector: pFastBac
Product Type: Baculovirus Expression System
Protein Tag: Untagged
Quantity: 1 kit
Product Line: Bac-to-Bac™
Promoter:
Polyhedrin
Content And Storage:
Each Bac-to-Bac Baculovirus Expression System includes:• pFastBac1 vector (10 μg), store at -20°C• pFastBac 1-Gus control vector (4 ng), store at -20°C• MAX Efficiency DH10Bac Chemically CompetentE. coli(1 kit with 5 x 0.1 mL), store at -80°C• ExpiFectamine Sf Transfection Reagent (1 mL), store at 4°C, do not freeze
Cloning Method:
Restriction Enzyme/MCS
Vector:
pFastBac
Product Type:
Baculovirus Expression System
Protein Tag:
Untagged
Quantity:
1 kit
Product Line:
Bac-to-Bac™
Promoter: __
Content And Storage: __
Cloning Method: __
Vector: __
Product Type: __
Protein Tag: __
Quantity: 6 Assays
Product Line: __
Promoter:
__
Content And Storage:
__
Cloning Method:
__
Vector:
__
Product Type:
__
Protein Tag:
__
Quantity:
6 Assays
Product Line:
__
Promoter: __
Content And Storage: Contains:• MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 μl each (total of 500 μl)• pUC19 DNA (0.01 μg/ml): 1 vial, 100 μl• SOC Medium: 2 bottles, 6 ml eachStore Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Cloning Method: __
Vector: __
Product Type: Competent Cells
Protein Tag: __
Quantity: 5 x 100 μL
Product Line: DH10Bac, MAX Efficiency™
Promoter:
__
Content And Storage:
Contains:• MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 μl each (total of 500 μl)• pUC19 DNA (0.01 μg/ml): 1 vial, 100 μl• SOC Medium: 2 bottles, 6 ml eachStore Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Cloning Method:
__
Vector:
__
Product Type:
Competent Cells
Protein Tag:
__
Quantity:
5 x 100 μL
Product Line:
DH10Bac, MAX Efficiency™
Promoter: __
Content And Storage: Contains one tube (1 ml) Cellfectin™ II Reagent. Store at 4°C. Do not freeze.
Cloning Method: __
Vector: __
Product Type: Reagent
Protein Tag: __
Quantity: 1 mL
Product Line: Cellfectin™
Promoter:
__
Content And Storage:
Contains one tube (1 ml) Cellfectin™ II Reagent. Store at 4°C. Do not freeze.
Cloning Method:
__
Vector:
__
Product Type:
Reagent
Protein Tag:
__
Quantity:
1 mL
Product Line:
Cellfectin™