Invitrogen™ T-REx™ Core Kit, with pcDNA™4/TO/myc-His© Vector

Description

• Uses complete CMV promoter and adds control elements from bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of strongest mammalian promoter sequences known Specific Activation: uses repressor mechanism that blocks transcription from powerful CMV promoter in absence of tetracycline Because T-REx System elements do not use viral transactivators, can achieve high-level expression from complete CMV promoter without secondary, non-specific activation of host genes Two tetracycline operator sequences (TetO 2 ) have been inserted between TATA box of CMV promoter and transcriptional start site TetO 2 sequence itself has no effect on expression: When tetracycline repressor protein (TR) is present, it effectively binds TetO 2 sites and blocks transcription initiation Tetracycline added to culture medium binds to, and changes conformation of, TR protein causing TR protein to release TetO 2 sites and derepressing transcription from CMV promoter: The result is high-level expression of gene of interest Expression levels can be modulated based on tetracycline concentration and can be induced to levels that are achieved with constitutive CMV expression vectors Complete CMV enhancer-promoter sequence containing two copies of tetracycline operator TetO 2 sequence for high-level regulated expression Zeocinor hygromycin resistance gene for effective selection of stable mammalian cell lines Large multiple cloning site to simplify cloning In addition, pcDNA™4/TO/ myc -His offers a c-myc epitope for rapid detection of recombinant protein with Anti- myc Antibody and polyhistidine (6xHis) sequence for simple purification of recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody Regulatory vector, pcDNA™6/TR, is provided for high-level expression of tetracycline repressor (TR) protein Vector expresses Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express TR protein Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression