Invitrogen™ pDisplay™ Mammalian Expression Vector

Description

• Displayed proteins can be analyzed for their ability to interact with known or putative ligands Proteins of interest are targeted and anchored to cell surface by cloning the gene of interest in frame with vectors unique N-terminal secretion signal and C-terminal transmembrane anchoring domain of platelet-derived growth factor receptor (PDGFR) In contrast to phage display vectors which operate exclusively in prokaryotic cells, pDisplay vector offers advantage of having displayed protein of interest processed in mammalian cells Therefore, recombinant proteins of eukaryotic origin that are expressed from pDisplay more closely resemble their native form: this may favor more accurate ligand binding interactions In addition to N-terminal cell surface targeting signal and C-terminal transmembrane anchoring domain, pDisplay vector contains T7 promoter/priming site for in vitro transcription of sense RNA and for sequencing of inserts Neomycin resistance marker for stable selection in mammalian cells using Geneticin™ SV40 origin for replication and simple vector rescue in cell lines expressing large T antigen (e.g.,COS-1 and COS-7) Ampicillin resistance gene for selection in E
• coli Mammalian Two-Hybrid, Protein-Protein Interactions, Proteins, Expression, Isolation and Analysis