QuantiGene™ Plex Assay Kit

Description

• The QuantiGene Plex Assay Kit contains the generic reagents, buffers, and plates necessary to perform the assay in 96-well plates, and must be used with a target-specific QuantiGene Plex panel (sold separately)
• The QuantiGene Plex Assay is a gene expression assay that enables multiplexed measurement of up to 80 RNA transcript targets
• The assay uses target-specific probes to capture the RNA of interest, and branched DNA technology then amplifies fluorescent signals which are read with a Luminex xMAP instrument
• • Difficult sample types —works with degraded and cross-linked RNA in FFPE tissues and blood • True multiplexing —measure up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity • Standardized platform —uses 96 or 384-well plate formats compatible with Luminex 200, MagPix, and FlexMap3D systems • Simple workflow —employs an ELISA-like workflow for direct hybridization of transcripts to beads and transcript labeling • Large inventory of validated genes —select from over 15,000 genes to create pathway and disease-themed panels • Rapid turnaround for custom panels —if we don’t have your gene(s), we can create a custom panel within two weeks Technology QuantiGene assays utilize branched DNA (bDNA) technology that has been used for decades in the VERSANT™ diagnostic assays for HIV-1, HCV, and HBV
• First, cells are lysed or tissue samples are homogenized to release the target RNA
• Second, the oligonucleotide probe set is incubated with the target RNA and Luminex xMAP bead set overnight
• During this incubation, the probes cooperatively hybridize to the target and capture probes bound to each bead, capturing the target RNA
• Third, signal amplification is performed via sequential hybridization of the bDNA preamplifier, amplifier, and label-probe molecules to the target
• Addition of streptavidin phycoerythrin generates a luminescent signal directly proportional to the amount of target mRNA present in the sample
• Applications QuantiGene Plex assay applications include compound screening of drug candidates, biomarker verification, siRNA knockdown efficiency, prospective and/or retrospective analysis of clinical samples, miRNA profiling, microarray validation, predictive toxicology, and detection of translocations and fusion genes.