Invitrogen™ Lyo-ready Bst DNA Polymerase

Description

• Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors
• By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP)
• Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors
• By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP)
• Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity
• It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies
• Lyo-ready Bst DNA Polymerase features • Fast target detection in as little as 10 minutes • Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction • Robust amplification, even from inhibitor-containing RNA or DNA samples • Ability to optimize LAMP or RT-LAMP reactions to fit your needs Applications Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including: • Loop-mediated isothermal amplification (LAMP) • Whole genome amplification (WGA) • Rolling circle amplification (RCA) • Recombinase polymerase amplification (RPA) • Helicase-dependent amplification (HDA) • Exponential amplification reaction (EXPAR) Notes 1
• If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use
• 2
• SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.