MilliporeSigma™ CpG MethylQuest™ Protein (Recombinant GST-MBD fusion protein)

Description

• The CpG MethylQuest protein is an effective and versatile tool for the study of CpG methylation in DNA
• This purified recombinant protein contains the methyl binding domain (MBD) of the mouse MBD2b protein fused to a glutathione-S-transferase protein (GST) from S japonicum
• These two protein domains are separated by a linker containing a thrombin cleavage site
• This unbound protein has several unique properties that can be incorporated into your own customized assays for the study of DNA methylation
• Competitive elution of the GST-MBD protein or GST-MBD-methylated DNA complexes with glutathione
• Elution of intact complexes by thrombin digestion Immobilization of protein DNA complexes or GST-MBD protein to other solid surfaces such as microtiter plates coated with GST antibodies
• Visualization of complexes using labeled GST antibodies or GST antibodies and labeled secondary antibodies
• Surface cysteine groups on the GST protein can be chemically modified to allow the addition of other reporters, affinity tags, or functional groups
• To simplify the isolation of methylated DNA, this protein can also be obtained pre-bound to magnetic beadsas a component of the CpG MethylQuest DNA Isolation Kit (17-10035).