4530700

Thermo Scientific™ IPTG, dioxane-free

Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.

Manufacturer: Fischer Scientific

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Description

  • IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose
  • It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization
  • IPTG is used to induce expression of cloned genes under control of the lac operon
  • It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening
  • It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization
  • IPTG is used to induce expression of cloned genes under control of the lac operon
  • It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening
  • Ready-to-use 100mM IPTG aqueous solution is non-toxic, stable, 0.22μm membrane-filtered, formulated for direct use in blue/white colony screening.