Invitrogen™ Linear Acrylamide (5 mg/ml) (1 ml Tube)

Description

• Invitrogen Linear Acrylamide (5 mg/mL) is for use as a nucleic acid coprecipitant and is ideal for PCR and RT-PCR reactions since small amounts of contaminating nucleic acids present in other carriers could be amplified
• Ideal for RT-PCR Increases pellet mass Quantitative recovery of low concentrations (ng/mL) of nucleic acid Prevents pellet loss in nuclease protection assays What is a Coprecipitant? Co-precipitants are inert substances used to aid recovery of nucleic acids during alcohol precipitations
• While they can be used for precipitating large amounts of nucleic acids, they are essential for quantitative recovery of small amounts of nucleic acids in dilute solutions
• Often, the use of such molecules is desirable for no other reason but visualization of the pelleted precipitate after centrifugation
• Linear acrylamide offers advantages over other coprecipitants in that it is chemically synthesized and not treated with reagents (e.g., proteases) derived from biological sources
• Therefore, it is not contaminated with biological material making it ideal for use upstream of RT-PCR
• It has been shown to precipitate pictogram amounts of DNA fragments larger than 20 base pairs while failing to precipitate shorter fragments and free nucleotides
• Linear acrylamide is useful for separating reaction products from unincorporated nucleotides and from most oligonucleotide primers
• It should be used at a final working concentration of 10 to 20 μg/mL
• Linear acrylamide will not interfere with A 260/280 readings
• Supplied in 5 x 1 mL tubes
• Order Info Shipping Condition: Dry Ice