Thermo Scientific™ X-Gal Solution, ready-to-use

Description

• Formula: C 14 H 15 BrClNO 6 Molecular weight: 408.6 X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate
• Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies ( see Supporting data)
• X-Gal Solution, ready-to-use, is stable, 0.22 μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening
• Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies
• X-Gal Solution, ready-to-use, is a stable, 0.22μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening
• Recommended for: Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1); Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells; Detection of beta-galactosidase activity in immunological and histochemical procedures
• Note: Preparation of a 20mg/mL stock solution in dimethylformamide (DMF) or dimethylsulfoxide (DMSO) is recommended for X-Gal
• DMF dissolves some plastic materials
• The direct addition of DMF-containing solution to plastic Petri dishes should be avoided
• DMF is toxic
• Allow X-Gal solution containing dimethylformamide to completely dry before plating bacteria on agar plates.