4530697

Thermo Scientific™ X-Gluc

Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded bygusA, a widely used reporter gene.

Manufacturer: Fischer Scientific

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Detection Method

Colorimetric

Product Type

X-Gluc

Substrate Properties

Chemical Substrate

Substrate Type

Beta-Glucuronidase (GUS) Substrate

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Description

  • X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA , a widely used reporter gene
  • Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo ( see the scheme below)
  • Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo ( see the scheme below)
  • Recommended for: Detection of GUS expression in plant cells and tissues (1) Detection of infections caused by E.coli Detection of bacterial contamination in food and water samples Note: Preparation of a 0.1M stock solution in dimethylformamide or dimethylsulfoxide is recommended.

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