Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 20-158-MI-Each-of-1 | In Stock | ₹ 83,634.90 |
20-158-MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 83,634.90
GST (18%): ₹ 15,054.282
Total Price: ₹ 98,689.182
Assay
EMSA Assay
Detection Method
Chemiluminescence
Sample Type
Protein
No. of Reactions
100 reactions
Product Type
LightShift Chemiluminescent RNA EMSA Kit
Technique
Enhanced Chemiluminescence, Membrane Blot, Gel Shift
Content And Storage
Sufficient For: 100 binding reactions and detection on 1000 cm2of membrane (10 mini-gel blots)• 125 nM Biotinylated IRE RNA Control 35 μL (store at -20°C)• 10 μM Unlabeled IRE RNA Control, 25 μL (store at -20°C)• 2 mg/mL Cytosolic Liver Extract, 50 μL (store at -20°C)• 10 mg/mL tRNA, 100 μL (store at -20°C)• 10X REMSA Binding Buffer, 1 mL (store at -20°C)• 50% Glycerol, 500 μL (store at -20°C)• 2M KCl, 500 μL (store at -20°C)• 1M MgCl2, 500 μL (store at -20°C)• DTT, lyophilized (store at -20°C)• Nuclease-Free Water, 1.5 mL (store at -20°C)• 5X REMSA Loading Buffer, 1 mL (store at -20°C)• Stabilized Streptavidin-HRP, 1.5 mL (store at 4°C)• Luminol/Enhancer Solution, 80 mL (store at 4°C)• Stable Peroxide Solution, 80 mL (store at 4°C)• Nucleic Acid Detection Blocking Buffer, 500 mL (store at 4°C)• 4X Wash Buffer, 500 mL (store at 4°C)• Substrate Equilibration Buffer, 500 mL (store at 4°C)
Format
Kit
For Use With (Equipment)
myECL™ Imager, X-Ray Film
Product Line
LightShift™
Target Specificity
Not Target-Specific
Related Products
Description
- Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA)
- The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32 P-isotopic methods
- The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction
- Highlights: Sensitive – chemiluminescent detection comparable to radioactive detection Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems Flexible – compatible with RNA probes biotinylated by different methods Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection Non-radioactive – eliminate waste concerns from radioactive RNA probes
Compare Similar Items
Show Difference
Assay: EMSA Assay
Detection Method: Chemiluminescence
Sample Type: Protein
No. of Reactions: 100 reactions
Product Type: LightShift Chemiluminescent RNA EMSA Kit
Technique: Enhanced Chemiluminescence, Membrane Blot, Gel Shift
Content And Storage: Sufficient For: 100 binding reactions and detection on 1000 cm2of membrane (10 mini-gel blots)• 125 nM Biotinylated IRE RNA Control 35 μL (store at -20°C)• 10 μM Unlabeled IRE RNA Control, 25 μL (store at -20°C)• 2 mg/mL Cytosolic Liver Extract, 50 μL (store at -20°C)• 10 mg/mL tRNA, 100 μL (store at -20°C)• 10X REMSA Binding Buffer, 1 mL (store at -20°C)• 50% Glycerol, 500 μL (store at -20°C)• 2M KCl, 500 μL (store at -20°C)• 1M MgCl2, 500 μL (store at -20°C)• DTT, lyophilized (store at -20°C)• Nuclease-Free Water, 1.5 mL (store at -20°C)• 5X REMSA Loading Buffer, 1 mL (store at -20°C)• Stabilized Streptavidin-HRP, 1.5 mL (store at 4°C)• Luminol/Enhancer Solution, 80 mL (store at 4°C)• Stable Peroxide Solution, 80 mL (store at 4°C)• Nucleic Acid Detection Blocking Buffer, 500 mL (store at 4°C)• 4X Wash Buffer, 500 mL (store at 4°C)• Substrate Equilibration Buffer, 500 mL (store at 4°C)
Format: Kit
For Use With (Equipment): myECL™ Imager, X-Ray Film
Product Line: LightShift™
Target Specificity: Not Target-Specific
Assay: __
Detection Method: Chemiluminescence
Sample Type: __
No. of Reactions: __
Product Type: tRNA for LightShift RNA EMSA Kit
Technique: __
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -70°C.
Format: Kit
For Use With (Equipment): __
Product Line: LightShift™
Target Specificity: Not Target-Specific
Assay: __
Detection Method: __
Sample Type: __
No. of Reactions: __
Product Type: __
Technique: __
Content And Storage: __
Format: __
For Use With (Equipment): __
Product Line: __
Target Specificity: __