PI20148X
Thermo Scientific™ LightShift™ EMSA Optimization and Control Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI20148X-Each-of-1 | In Stock | ₹ 37,518.06 |
PI20148X - Each of 1
In Stock
Quantity
1
Base Price: ₹ 37,518.06
GST (18%): ₹ 6,753.251
Total Price: ₹ 44,271.311
Assay
EMSA Assay
Format
Kit
For Use With (Equipment)
myECL™ Imager, X-Ray Film
Product Type
LightShift EMSA Optimization and Control Kit
Target Specificity
Not Target-Specific
Content And Storage
Sufficient For: 100 binding reactions• 10X Binding Buffer, 1 mL• Biotin-EBNA Control DNA, 50 μL• Unlabeled EBNA DNA, 50 μL• EBNA Extract, 125 μL• Poly (dI·dC), 125 μL• 50% Glycerol, 500 μL• 1% NP-40, 500 μL• 1 M KCl, 1 mL• 100mM MgCl2, 500 μL• 200mM EDTA pH 8.0, 500 μL• 5X Loading Buffer, 1 mLStore at -20°C.
Includes
10X Binding Buffer (1 mL), Biotin-EBNA Control DNA (50 μL), Unlabeled EBNA DNA (50 μL), EBNA Extract (125 μL), Poly dI·dC (125 μL), 50% Glycerol (500 μL), 1% NP-40 (500 μL), 1M KCl (1 mL), 100mM MgCl2(500 μL), 200mM EDTA pH 8.0 (500 μL), 5X Loading Buffer (1 mL)
Product Line
LightShift™
Quantity
100 Reactions
Technique
Gel Shift
Related Products
Description
- Thermo Scientific™ LightShift Chemiluminescent EMSA Kit is an extraordinarily robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions
- The kit includes reagents for setting up and customizing protein:DNA binding reactions, a control set of DNA and protein extract to test the kit system, stabilized streptavidin-HRP conjugate to probe for the biotin-labeled DNA target, and an exceptionally sensitive chemiluminescent substrate module for detection.The principle for LightShift™ EMSA Detection is similar to a Western blot
- Biotin end-labeled duplex DNA is incubated with a nuclear extract or purified factor and electrophoresed on a native gel
- The DNA is then rapidly (30 minutes) transferred to a positive nylon membrane, UV-crosslinked, probed with streptavidin-HRP conjugate and incubated with the substrate
- The protocol from labeling to results can be accomplished in a single day
- All you need to perform the assay are purified DNA target that has been end-labeled with biotin, the protein extract you wish to test, nylon membrane and basic electrophoresis equipment
- DNA targets may be synthesized with 5' or 3' biotin labels or they may be labeled after synthesis using the Thermo Scientific Biotin 3' End DNA Labeling Kit (Part No
- 89818)
- Nuclear, cytosolic or whole cell protein extracts may be obtained by a variety of methods, including the Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Part No
- 78833)
- Highlights: Includes EBNA control system to help new users develop a working assay and understand the methods used to confirm binding interaction specificity Excellent for detecting low-abundance proteins in nuclear extracts Sensitivity that surpasses radioactive and digoxigenin methods Compatible with previously established binding conditions for popular DNA:protein interactions
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Assay: EMSA Assay
Format: Kit
For Use With (Equipment): myECL™ Imager, X-Ray Film
Product Type: LightShift EMSA Optimization and Control Kit
Target Specificity: Not Target-Specific
Content And Storage: Sufficient For: 100 binding reactions• 10X Binding Buffer, 1 mL• Biotin-EBNA Control DNA, 50 μL• Unlabeled EBNA DNA, 50 μL• EBNA Extract, 125 μL• Poly (dI·dC), 125 μL• 50% Glycerol, 500 μL• 1% NP-40, 500 μL• 1 M KCl, 1 mL• 100mM MgCl2, 500 μL• 200mM EDTA pH 8.0, 500 μL• 5X Loading Buffer, 1 mLStore at -20°C.
Includes: 10X Binding Buffer (1 mL), Biotin-EBNA Control DNA (50 μL), Unlabeled EBNA DNA (50 μL), EBNA Extract (125 μL), Poly dI·dC (125 μL), 50% Glycerol (500 μL), 1% NP-40 (500 μL), 1M KCl (1 mL), 100mM MgCl2(500 μL), 200mM EDTA pH 8.0 (500 μL), 5X Loading Buffer (1 mL)
Product Line: LightShift™
Quantity: 100 Reactions
Technique: Gel Shift
Assay:
EMSA Assay
Format:
Kit
For Use With (Equipment):
myECL™ Imager, X-Ray Film
Product Type:
LightShift EMSA Optimization and Control Kit
Target Specificity:
Not Target-Specific
Content And Storage:
Sufficient For: 100 binding reactions• 10X Binding Buffer, 1 mL• Biotin-EBNA Control DNA, 50 μL• Unlabeled EBNA DNA, 50 μL• EBNA Extract, 125 μL• Poly (dI·dC), 125 μL• 50% Glycerol, 500 μL• 1% NP-40, 500 μL• 1 M KCl, 1 mL• 100mM MgCl2, 500 μL• 200mM EDTA pH 8.0, 500 μL• 5X Loading Buffer, 1 mLStore at -20°C.
Includes:
10X Binding Buffer (1 mL), Biotin-EBNA Control DNA (50 μL), Unlabeled EBNA DNA (50 μL), EBNA Extract (125 μL), Poly dI·dC (125 μL), 50% Glycerol (500 μL), 1% NP-40 (500 μL), 1M KCl (1 mL), 100mM MgCl2(500 μL), 200mM EDTA pH 8.0 (500 μL), 5X Loading Buffer (1 mL)
Product Line:
LightShift™
Quantity:
100 Reactions
Technique:
Gel Shift
Assay: __
Format: __
For Use With (Equipment): __
Product Type: Detergent
Target Specificity: __
Content And Storage: Store at Room Temperature
Includes: __
Product Line: Brij™, Surfact-Amps™
Quantity: 950 mL
Technique: __
Assay:
__
Format:
__
For Use With (Equipment):
__
Product Type:
Detergent
Target Specificity:
__
Content And Storage:
Store at Room Temperature
Includes:
__
Product Line:
Brij™, Surfact-Amps™
Quantity:
950 mL
Technique:
__
Assay: __
Format: Kit
For Use With (Equipment): __
Product Type: RNA Labeling Kit
Target Specificity: __
Content And Storage: Sufficient For: 20 desthiobiotinylation reactions using 50 pmol of RNA each• Desthiobiotinylated Cytidine Bisphosphate, 40 μL• T4 RNA Ligase, 40 μL• T4 RNA Ligase Reaction buffer (10X), 100 μL• Non-labeled RNA Control, 100 μL• Biotinylated IRE RNA Control, 35 μL• RNase Inhibitor, 2 x 10 μL• DMSO, 200 μL• PEG (30%), 300 μL• Glycogen, 20 μL• Nuclease-free Water, 1.5 mLUpon receipt, store at -20°C.
Includes: __
Product Line: Pierce™
Quantity: 20 Reactions
Technique: __
Assay:
__
Format:
Kit
For Use With (Equipment):
__
Product Type:
RNA Labeling Kit
Target Specificity:
__
Content And Storage:
Sufficient For: 20 desthiobiotinylation reactions using 50 pmol of RNA each• Desthiobiotinylated Cytidine Bisphosphate, 40 μL• T4 RNA Ligase, 40 μL• T4 RNA Ligase Reaction buffer (10X), 100 μL• Non-labeled RNA Control, 100 μL• Biotinylated IRE RNA Control, 35 μL• RNase Inhibitor, 2 x 10 μL• DMSO, 200 μL• PEG (30%), 300 μL• Glycogen, 20 μL• Nuclease-free Water, 1.5 mLUpon receipt, store at -20°C.
Includes:
__
Product Line:
Pierce™
Quantity:
20 Reactions
Technique:
__
Assay: __
Format: Bottle
For Use With (Equipment): __
Product Type: __
Target Specificity: __
Content And Storage: 2 mL of settled resin. Provided as 8 mL of 25% slurry.Store at 4°C. Do not freeze.
Includes: __
Product Line: Pierce™
Quantity: 2 mL
Technique: __
Assay:
__
Format:
Bottle
For Use With (Equipment):
__
Product Type:
__
Target Specificity:
__
Content And Storage:
2 mL of settled resin. Provided as 8 mL of 25% slurry.Store at 4°C. Do not freeze.
Includes:
__
Product Line:
Pierce™
Quantity:
2 mL
Technique:
__