20-160-MI
Thermo Scientific™ Pierce™ RNA 3' End Biotinylation Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 20-160-MI-Each-of-1 | In Stock | ₹ 85,902.24 |
20-160-MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 85,902.24
GST (18%): ₹ 15,462.403
Total Price: ₹ 1,01,364.643
Content And Storage
Sufficient For: 20 labeling reactions (each with 50 pmol RNA)• T4 RNA Ligase Reaction Buffer (10X), 100 μL• T4 RNA Ligase (20,000 U/mL), 40 μL• Non-labeled RNA Control (10 μM), 100 μL• Biotinylated IRE RNA Control (125 nM), 35 μL• RNase Inhibitor (40 U/μL), 2 × 10 μL• Nuclease-free Water, 1.5 mL• DMSO, 200 μL• 30% PEG, 300 μL• Biotinylated Cytidine (Bis)phosphate (1mM), 40 μL• Glycogen (20 mg/mL), 20 μLStore all components at -20°C
Product Line
Pierce™
Quantity
20 Reactions
Format
Kit
Product Type
RNA Labeling Kit
Related Products
Description
- Thermo Scientific™ Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions
- The complete kit contains an unlabeled RNA strand to use as a positive control, a biotinylated-RNA probe standard, RNase inhibitor, glycogen and ligation-enhancing reagents
- The RNA 3' End Biotinylation Kit uses T4 RNA ligase to conjugate a single nucleotide analog to the 3' terminus of an RNA strand
- The unique biotinylated cytidine (bis)phosphate used in this kit enables T4 RNA ligase to attach a single biotin tag on RNA probes of diverse secondary structure and lengths with greater than 70% efficiency
- Highlights: Non-radioactive – incorporates a biotin label which allows detection sensitivity similar to radioactivity when used with chemiluminescent detection systems Fast – RNA can be labeled in 0.5-2 hours with minimal downstream processing Easy to use – RNA ligase and optimized reaction buffer are included Economical – only a fraction of the cost of purchasing synthetic biotinylated RNA probes End-labeled – results in minimal disturbance of RNA secondary structure Flexible – label synthetic or in vitro transcribed RNA probes ranging from 22 to 450 nucleotides
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Content And Storage: Sufficient For: 20 labeling reactions (each with 50 pmol RNA)• T4 RNA Ligase Reaction Buffer (10X), 100 μL• T4 RNA Ligase (20,000 U/mL), 40 μL• Non-labeled RNA Control (10 μM), 100 μL• Biotinylated IRE RNA Control (125 nM), 35 μL• RNase Inhibitor (40 U/μL), 2 × 10 μL• Nuclease-free Water, 1.5 mL• DMSO, 200 μL• 30% PEG, 300 μL• Biotinylated Cytidine (Bis)phosphate (1mM), 40 μL• Glycogen (20 mg/mL), 20 μLStore all components at -20°C
Product Line: Pierce™
Quantity: 20 Reactions
Format: Kit
Product Type: RNA Labeling Kit
Content And Storage:
Sufficient For: 20 labeling reactions (each with 50 pmol RNA)• T4 RNA Ligase Reaction Buffer (10X), 100 μL• T4 RNA Ligase (20,000 U/mL), 40 μL• Non-labeled RNA Control (10 μM), 100 μL• Biotinylated IRE RNA Control (125 nM), 35 μL• RNase Inhibitor (40 U/μL), 2 × 10 μL• Nuclease-free Water, 1.5 mL• DMSO, 200 μL• 30% PEG, 300 μL• Biotinylated Cytidine (Bis)phosphate (1mM), 40 μL• Glycogen (20 mg/mL), 20 μLStore all components at -20°C
Product Line:
Pierce™
Quantity:
20 Reactions
Format:
Kit
Product Type:
RNA Labeling Kit
Content And Storage: -20°C for 12 months
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Quantity: 10 mL
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-20°C for 12 months
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10 mL
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